| http://purl.uniprot.org/citations/20413728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20413728 | http://www.w3.org/2000/01/rdf-schema#comment | "Chemotaxis and integrin activation are essential processes for neutrophil transmigration in response to injury. CalDAG-GEFI plays a key role in the activation of beta1, beta2, and beta3 integrins in platelets and neutrophils by exchanging a GDP for a GTP on Rap1. Here, we explored the role of CalDAG-GEFI and Rap1b in integrin-independent neutrophil chemotaxis. In a transwell assay, CalDAG-GEFI-/-neutrophils had a 46% reduction in transmigration compared with WT in response to a low concentration of LTB4. Visualization of migrating neutrophils in the presence of 10 mM EDTA revealed that CalDAG-GEFI-/-neutrophils had abnormal chemotactic behavior compared with WT neutrophils, including reduced speed and directionality. Interestingly, Rap1b-/-neutrophils had a similar phenotype in this assay, suggesting that CalDAG-GEFI may be acting through Rap1b. We investigated whether the deficit in integrin-independent chemotaxis in CalDAG-GEFI-/-neutrophils could be explained by defective cytoskeleton rearrangement. Indeed, we found that CalDAG-GEFI-/-neutrophils had reduced formation of F-actin pseudopodia after LTB4 stimulation, suggesting that they have a defect in polarization. Overall, our studies show that CalDAG-GEFI helps regulate neutrophil chemotaxis, independent of its established role in integrin activation, through a mechanism that involves actin cytoskeleton and cellular polarization."xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.org/dc/terms/identifier | "doi:10.1189/jlb.0110049"xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/author | "Hattori H."xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/author | "Sakai J."xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/author | "Wagner D.D."xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/author | "Luo H.R."xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/author | "Duerschmied D."xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/author | "Cifuni S.M."xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/author | "Goerge T."xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/author | "White G.C."xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/author | "Chrzanowska-Wodnicka M."xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/author | "Carbo C."xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/name | "J Leukoc Biol"xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/pages | "313-319"xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/title | "Integrin-independent role of CalDAG-GEFI in neutrophil chemotaxis."xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://purl.uniprot.org/core/volume | "88"xsd:string |
| http://purl.uniprot.org/citations/20413728 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20413728 |
| http://purl.uniprot.org/citations/20413728 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20413728 |
| http://purl.uniprot.org/uniprot/#_D3YYP8-mappedCitation-20413728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20413728 |
| http://purl.uniprot.org/uniprot/#_D3Z1S4-mappedCitation-20413728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20413728 |
| http://purl.uniprot.org/uniprot/#_D3Z2F9-mappedCitation-20413728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20413728 |
| http://purl.uniprot.org/uniprot/#_D3YXD6-mappedCitation-20413728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20413728 |
| http://purl.uniprot.org/uniprot/#_A0A1W2P777-mappedCitation-20413728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20413728 |