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http://purl.uniprot.org/citations/20416094http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20416094http://www.w3.org/2000/01/rdf-schema#comment"

Background

Tissue fibrosis is an integral component of chronic inflammatory (liver and pancreas) diseases and pancreatic cancer. Activated pancreatic- (PSC) and hepatic-(HSC) stellate cells play a key role in fibrogenesis. To identify organ- and disease-specific stellate cell transcriptional fingerprints, we employed genome-wide transcriptional analysis of primary human PSC and HSC isolated from patients with chronic inflammation or cancer.

Methods

Stellate cells were isolated from patients with pancreatic ductal adenocarcinoma (n = 5), chronic pancreatitis (n = 6), liver cirrhosis (n = 5) and liver metastasis of pancreatic ductal adenocarcinoma (n = 6). Genome-wide transcriptional profiles of stellate cells were generated using our 51K human cDNA microarray platform. The identified organ- and disease specific genes were validated by quantitative RT-PCR, immunoblot, ELISA, immunocytochemistry and immunohistochemistry.

Results

Expression profiling identified 160 organ- and 89 disease-specific stellate cell transcripts. Collagen type 11a1 (COL11A1) was discovered as a novel PSC specific marker with up to 65-fold higher expression levels in PSC compared to HSC (p < 0.0001). Likewise, the expression of the cytokine CCL2 and the cell adhesion molecule VCAM1 were confined to HSC. PBX1 expression levels tend to be increased in inflammatory- vs. tumor-stellate cells. Intriguingly, tyrosine kinase JAK2 and a member of cell contact-mediated communication CELSR3 were found to be selectively up-regulated in tumor stellate cells.

Conclusions

We identified and validated HSC and PSC specific markers. Moreover, novel target genes of tumor- and inflammation associated stellate cells were discovered. Our data may be instrumental in developing new tailored organ- or disease-specific targeted therapies and stellate cell biomarkers."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.org/dc/terms/identifier"doi:10.1186/1476-4598-9-88"xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Ansorge W."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Jiang X."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Pan Z."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Schwager C."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Weis N."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Wirkner U."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Friess H."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Abdollahi A."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Erkan M."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Kleeff J."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Giese N.A."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Debus J."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Huber P.E."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/author"Samkharadze T."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/name"Mol Cancer"xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/pages"88"xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/title"Organ-, inflammation- and cancer specific transcriptional fingerprints of pancreatic and hepatic stellate cells."xsd:string
http://purl.uniprot.org/citations/20416094http://purl.uniprot.org/core/volume"9"xsd:string
http://purl.uniprot.org/citations/20416094http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20416094
http://purl.uniprot.org/citations/20416094http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/20416094
http://purl.uniprot.org/uniprot/#_B4DSQ9-mappedCitation-20416094http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20416094
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