| http://purl.uniprot.org/citations/20432435 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20432435 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20432435 | http://www.w3.org/2000/01/rdf-schema#comment | "The stabilization of cell surface E-cadherin is important for the maintenance of apical junction complexes and epithelial polarity. Previously, we reported that discoidin domain receptor 1 (DDR1) forms a complex with E-cadherin at adhesive contacts; however, the regulatory role of DDR1 in the stabilization of cell surface E-cadherin and E-cadherin-mediated cell behaviors remained undefined. To gain insight into these questions, we utilized two stable clones depleted for DDR1 via the small interfering RNA (siRNA) technique, and we over-expressed DDR1 in MDCK cells. We performed Western blotting, immunofluorescence analysis, flow cytometry, and cell aggregation studies to investigate the effect of DDR1 on cell surface E-cadherin. The results showed that both DDR1/2 and E-cadherin use their extracellular domains to form DDR/E-cadherin complexes. Neither the depletion nor the over-expression of DDR1 changed the expression level of E-cadherin in MDCK cells. Collagen disrupted the formation of E-cadherin complexes and caused E-cadherin to accumulate in the cytoplasm; however, over-expression of DDR1 stabilized E-cadherin on the cell surface and decreased its cytoplasmic accumulation. Furthermore, independently of collagen stimulation, the depletion of DDR1 resulted in a decrease in the level of cell surface E-cadherin, which consequently caused its cytoplasmic accumulation and decreased E-cadherin-mediated cell aggregation. These results indicate that DDR1 can increase the stability of cell surface E-cadherin and promote MDCK cell aggregation, which may be mediated through the formation of DDR1/E-cadherin complexes. Overall, these findings have implications for the physiological roles of DDR1 in association with the maintenance of both the adhesion junction and epithelial polarity."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcp.22134"xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcp.22134"xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Chen W.C."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Chen W.C."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Wang C.K."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Wang C.K."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Eswaramoorthy R."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Eswaramoorthy R."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Ho M.L."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Ho M.L."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Hwang C.C."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Hwang C.C."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Tang M.J."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Tang M.J."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Wang C.Z."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Wang C.Z."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Wang H.M."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/author | "Wang H.M."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/name | "J. Cell. Physiol."xsd:string |
| http://purl.uniprot.org/citations/20432435 | http://purl.uniprot.org/core/name | "J. Cell. Physiol."xsd:string |