| http://purl.uniprot.org/citations/20439988 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20439988 | http://www.w3.org/2000/01/rdf-schema#comment | "Chondroitin sulfate (CS) and dermatan sulfate (DS) containing N-acetylgalactosamine 4,6-bissulfate (GalNAc(4,6-SO(4))) show various physiological activities through interacting with numerous functional proteins. N-Acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST) transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 6 of N-acetylgalactosamine 4-sulfate in CS or DS to yield GalNAc(4,6-SO(4)) residues. We here report generation of transgenic mice that lack GalNAc4S-6ST. GalNAc4S-6ST-null mice were born normally and fertile. In GalNAc4S-6ST-null mice, GalNAc(4,6-SO(4)) residues in CS and DS disappeared completely, indicating that GalNAc4S-6ST should be a sole enzyme responsible for the synthesis of GalNAc(4,6-SO(4)) residues in both CS and DS. IdoA-GalNAc(4,6-SO(4)) units that account for approximately 40% of total disaccharide units of DS in the liver of the wild-type mice disappeared in the liver DS of GalNAc4S-6ST-null mice without reduction of IdoA content. Bone marrow-derived mast cells (BMMCs) derived from GalNAc4S-6ST-null mice contained CS without GlcA-GalNAc(4,6-SO(4)) units. Tryptase and carboxypeptidase A activities of BMMCs derived from GalNAc4S-6ST-null mice were lower than those activities of BMMCs derived from wild-type mice, although mRNA expression of these mast cell proteases was not altered. Disaccharide compositions of heparan sulfate/heparin contained in the mast cells derived from BMMCs in the presence of stem cell factor were much different from those of heparan sulfate/heparin in BMMCs but did not differ significantly between wild-type mice and GalNAc4S-6ST-null mice. These observations suggest that CS containing GalNAc(4,6-SO(4)) residues in BMMCs may contribute to retain the active proteases in the granules of BMMCs but not for the maturation of BMMCs into connective tissue-type mast cells."xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.084749"xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/author | "Ito T."xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/author | "Kondo S."xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/author | "Kakehi S."xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/author | "Ohta T."xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/author | "Kimata K."xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/author | "Habuchi H."xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/author | "Habuchi O."xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/author | "Ohtake-Niimi S."xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/pages | "20793-20805"xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/title | "Mice deficient in N-acetylgalactosamine 4-sulfate 6-o-sulfotransferase are unable to synthesize chondroitin/dermatan sulfate containing N-acetylgalactosamine 4,6-bissulfate residues and exhibit decreased protease activity in bone marrow-derived mast cells."xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://purl.uniprot.org/core/volume | "285"xsd:string |
| http://purl.uniprot.org/citations/20439988 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20439988 |
| http://purl.uniprot.org/citations/20439988 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20439988 |
| http://purl.uniprot.org/uniprot/#_Q91XQ5-mappedCitation-20439988 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20439988 |
| http://purl.uniprot.org/uniprot/Q91XQ5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20439988 |