| http://purl.uniprot.org/citations/20452978 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20452978 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20452978 | http://www.w3.org/2000/01/rdf-schema#comment | "Assembly of voltage-dependent Ca(2+) channels (VDCCs) with their associated proteins regulates the coupling of VDCCs with upstream and downstream cellular events. Among the four isoforms of the Rab3-interacting molecule (RIM1 to -4), we have previously reported that VDCC beta-subunits physically interact with the long alpha isoform of the presynaptic active zone scaffolding protein RIM1 (RIM1alpha) via its C terminus containing the C(2)B domain. This interaction cooperates with RIM1alpha-Rab3 interaction to support neurotransmitter exocytosis by anchoring vesicles in the vicinity of VDCCs and by maintaining depolarization-triggered Ca(2+) influx as a result of marked inhibition of voltage-dependent inactivation of VDCCs. However, physiological functions have not yet been elucidated for RIM3 and RIM4, which exist only as short gamma isoforms (gamma-RIMs), carrying the C-terminal C(2)B domain common to RIMs but not the Rab3-binding region and other structural motifs present in the alpha-RIMs, including RIM1alpha. Here, we demonstrate that gamma-RIMs also exert prominent suppression of VDCC inactivation via direct binding to beta-subunits. In the pheochromocytoma PC12 cells, this common functional feature allows native RIMs to enhance acetylcholine secretion, whereas gamma-RIMs are uniquely different from alpha-RIMs in blocking localization of neurotransmitter-containing vesicles near the plasma membrane. Gamma-RIMs as well as alpha-RIMs show wide distribution in central neurons, but knockdown of gamma-RIMs attenuated glutamate release to a lesser extent than that of alpha-RIMs in cultured cerebellar neurons. The results suggest that sustained Ca(2+) influx through suppression of VDCC inactivation by RIMs is a ubiquitous property of neurons, whereas the extent of vesicle anchoring to VDCCs at the plasma membrane may depend on the competition of alpha-RIMs with gamma-RIMs for VDCC beta-subunits."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.M110.101311"xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m110.101311"xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Miki T."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Miki T."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Mori Y."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Mori Y."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Yagi M."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Yagi M."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Mori E."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Mori E."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Akiyama S."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Akiyama S."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Campbell K.P."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Campbell K.P."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Nakao A."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Nakao A."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Wakamori M."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Wakamori M."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Beedle A.M."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Beedle A.M."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Uriu Y."xsd:string |
| http://purl.uniprot.org/citations/20452978 | http://purl.uniprot.org/core/author | "Uriu Y."xsd:string |