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http://purl.uniprot.org/citations/20465946http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20465946http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To study the role of Phosphatidylinositol 3 kinase (PI3K) in silica-induced DNA double strand break repair in human embryo lung fibroblasts (HELF).

Methods

Control HELF cells and DN-Deltap85 (HELF transfected with Dominant negative mutant of PI3K) were treated with 200 microg/ml silica for different times. The expression levels of phosphor-H2AX (H2AX), Ku70, Ku80 and DNA-PKcs were determined by Western blot. Furthermore, DNA double strand breaks were measured by neutral comet assay after cells were treated with 200 microg/ml silica for 0, 12 and 24 h.

Results

After treatment with 200 microg/ml silica for different times, the levels of H2AX were increased in a time-dependent manner and the expression levels of H2AX were obviously suppressed in DN-Deltap85 compared with control cells. The levels of Ku70 and Ku80 were also significantly suppressed in DN-Deltap85 (0.37 +/- 0.14, 0.55 +/-0.17) compared with control cells (0.58 +/- 0.09, 0.95 +/-0.21) after treatment with 200 microg/ml silica for 12 h (P < 0.05). Both the percentage of tail DNA in HELF and DN-Deltap85 increased significantly at 12 h (9.78 +/- 1.15, 11.79 +/-4.90) compared with groups without treatment with silica (2.40 +/-0.69, 3.31 +/- 1.35) and then decreased at 24 h (4.19 +/- 0.47, 7.58 +/-4.32), but only the decrease of HELF at 24 h was significant compared with HELF at 12 h (P < 0.05). DNA repair competence of HELF was 75.74% and that of DN-Deltap85 declined to 49.64%.

Conclusion

Silica dust can induce DNA double strand breaks in human embryo lung fibroblasts. PI3K might play a role in silica-induced DNA double strand break repair by regulating the expression levels of Ku70 and Ku80."xsd:string
http://purl.uniprot.org/citations/20465946http://purl.uniprot.org/core/author"Ye M."xsd:string
http://purl.uniprot.org/citations/20465946http://purl.uniprot.org/core/author"Liu B.C."xsd:string
http://purl.uniprot.org/citations/20465946http://purl.uniprot.org/core/author"Zhang F.M."xsd:string
http://purl.uniprot.org/citations/20465946http://purl.uniprot.org/core/author"Liu H.F."xsd:string
http://purl.uniprot.org/citations/20465946http://purl.uniprot.org/core/author"Jia X.W."xsd:string
http://purl.uniprot.org/citations/20465946http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20465946http://purl.uniprot.org/core/name"Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi"xsd:string
http://purl.uniprot.org/citations/20465946http://purl.uniprot.org/core/pages"241-245"xsd:string
http://purl.uniprot.org/citations/20465946http://purl.uniprot.org/core/title"[Roles of phosphatidylinositol 3 kinase in silica-induced DNA double strand breaks damage repair in human embryo lung fibroblasts]."xsd:string
http://purl.uniprot.org/citations/20465946http://purl.uniprot.org/core/volume"28"xsd:string
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