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http://purl.uniprot.org/citations/20470363http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20470363http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20470363http://www.w3.org/2000/01/rdf-schema#comment"

Background

Glycosyl transferases transfer glycosyl groups onto their substrate. Localization partially defines their function. Glycosyl transferase 25 domain 1 (GLT25D1) was recently shown to have galactosyltransferase activity towards collagens and another well known substrate, mannose binding lectin (MBL). To gain more insight in the role of galactosylation of lysines in the Gly-X-Lys repeats of collagenous proteins, we investigated the subcellular localization of GLT25D1.

Results

Immunofluorescence analysis of GLT25D1 expressed in the human hepatoma cell line (Huh7), revealed a perinuclear lattice like staining, resembling localization to the endoplasmic reticulum (ER). Possible targeting signals, an N-terminal signal sequence and a C-terminal ER-retention signal, were identified using prediction programs. These signals were then investigated by constructing a series of epitope-tagged forms of GLT25D1 that were analyzed by immunofluorescence and western blotting. In agreement with the predictions our results show that GLT25D1 is directed to the ER lumen as a soluble protein and retained there. Moreover, using two endoglycosidase enzymes EndoH and EndoF, we demonstrate that the putative bi-functional glycosyl transferase itself is a glycoprotein. Additionally we examined co-localization of GLT25D1 with MBL and lysyl hydroxylase 3 (LH3, PLOD3), which is a protein able to catalyze hydroxylation of lysine residues before they can be glycosylated. We demonstrate overlapping localization patterns of GLT25D1, MBL and LH3.

Conclusions

Taken together our data indicate that galactosylation of collagenous proteins by the soluble GLT25D1 occurs in the early secretory pathway."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.org/dc/terms/identifier"doi:10.1186/1471-2121-11-33"xsd:string
http://purl.uniprot.org/citations/20470363http://purl.org/dc/terms/identifier"doi:10.1186/1471-2121-11-33"xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/author"Spaan W.J."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/author"Spaan W.J."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/author"van Leeuwen H.C."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/author"van Leeuwen H.C."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/author"Liefhebber J.M."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/author"Liefhebber J.M."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/author"Punt S."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/author"Punt S."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/name"BMC Cell Biol."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/name"BMC Cell Biol."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/pages"33"xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/pages"33"xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/title"The human collagen beta(1-O)galactosyltransferase, GLT25D1, is a soluble endoplasmic reticulum localized protein."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/title"The human collagen beta(1-O)galactosyltransferase, GLT25D1, is a soluble endoplasmic reticulum localized protein."xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/volume"11"xsd:string
http://purl.uniprot.org/citations/20470363http://purl.uniprot.org/core/volume"11"xsd:string
http://purl.uniprot.org/citations/20470363http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20470363
http://purl.uniprot.org/citations/20470363http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20470363
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