| http://purl.uniprot.org/citations/20472831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20472831 | http://www.w3.org/2000/01/rdf-schema#comment | "The leukocyte integrin LFA-1 is critical for natural killer (NK) cell cytotoxicity as it mediates NK-cell adhesion to target cells and generates activating signals that lead to polarization of the actin cytoskeleton. However, the LFA-1-mediated signaling pathway is not fully understood. Here, we examined the subcellular localization of actin-associated proteins in wild-type, talin-deficient, and Wiskott-Aldrich Syndrome protein (WASP)-deficient NK cells bound to beads coated with the LFA-1 ligand intercellular adhesion molecule-1 (ICAM-1). In addition, we carried out coimmunoprecipitation analyses and also used a pharmacologic reagent to reduce the level of phosphatidylinositol-4,5-bisphosphate (PIP(2)). The results revealed the following signaling pathways. Upon ICAM-1 binding to LFA-1, talin redistributes to the site of LFA-1 ligation and initiates 2 signaling pathways. First, talin recruits the actin nucleating protein complex Arp2/3 via constitutive association of vinculin with talin and Arp2/3. Second, talin also associates with type I phosphatidylinositol 4-phosphate 5-kinase (PIPKI) and binding of LFA-1 to ICAM-1 results in localized increase in PIP(2). This increase in PIP(2) recruits WASP to the site of LFA-1 ligation where WASP promotes Arp2/3-mediated actin polymerization. These processes are critical for the initiation of NK cell-mediated cytotoxicity."xsd:string |
| http://purl.uniprot.org/citations/20472831 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2009-12-261487"xsd:string |
| http://purl.uniprot.org/citations/20472831 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/20472831 | http://purl.uniprot.org/core/author | "Siminovitch K.A."xsd:string |
| http://purl.uniprot.org/citations/20472831 | http://purl.uniprot.org/core/author | "Takei F."xsd:string |
| http://purl.uniprot.org/citations/20472831 | http://purl.uniprot.org/core/author | "Mace E.M."xsd:string |
| http://purl.uniprot.org/citations/20472831 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20472831 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/20472831 | http://purl.uniprot.org/core/pages | "1272-1279"xsd:string |
| http://purl.uniprot.org/citations/20472831 | http://purl.uniprot.org/core/title | "Elucidation of the integrin LFA-1-mediated signaling pathway of actin polarization in natural killer cells."xsd:string |
| http://purl.uniprot.org/citations/20472831 | http://purl.uniprot.org/core/volume | "116"xsd:string |
| http://purl.uniprot.org/citations/20472831 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20472831 |
| http://purl.uniprot.org/citations/20472831 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20472831 |
| http://purl.uniprot.org/uniprot/#_A2AIM2-mappedCitation-20472831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20472831 |
| http://purl.uniprot.org/uniprot/#_F6S1V7-mappedCitation-20472831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20472831 |
| http://purl.uniprot.org/uniprot/#_F6SX70-mappedCitation-20472831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20472831 |
| http://purl.uniprot.org/uniprot/#_Q53WY0-mappedCitation-20472831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20472831 |
| http://purl.uniprot.org/uniprot/#_P26039-mappedCitation-20472831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20472831 |
| http://purl.uniprot.org/uniprot/#_Q3UHS6-mappedCitation-20472831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20472831 |
| http://purl.uniprot.org/uniprot/#_Q0V930-mappedCitation-20472831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20472831 |
| http://purl.uniprot.org/uniprot/#_P70315-mappedCitation-20472831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20472831 |
| http://purl.uniprot.org/uniprot/#_Q3TBC3-mappedCitation-20472831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20472831 |
| http://purl.uniprot.org/uniprot/#_Q61078-mappedCitation-20472831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20472831 |
| http://purl.uniprot.org/uniprot/#_Q80TM2-mappedCitation-20472831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20472831 |