| http://purl.uniprot.org/citations/20479936 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20479936 | http://www.w3.org/2000/01/rdf-schema#comment | "Progranulin deficiency is thought to cause some forms of frontotemporal dementia (FTD), a major early-onset age-dependent neurodegenerative disease. How progranulin (PGRN) expression is regulated is largely unknown. We identified an evolutionarily conserved binding site for microRNA-29b (miR-29b) in the 3' untranslated region (3'UTR) of the human PGRN (hPGRN) mRNA. miR-29b downregulates the expression of luciferase through hPGRN or mouse PGRN (mPGRN) 3'UTRs, and the regulation was abolished by mutations in the miR-29b binding site. To examine the direct effect of manipulating endogenous miR-29b on hPGRN expression, we established a stable NIH3T3 cell line that expresses hPGRN under the control of the cytomegalovirus promoter. Ectopic expression of miR-29b decreased hPGRN expression at the both mRNA and protein levels. Conversely, knockdown of endogenous miR-29b with locked nucleic acid increased the production and secretion of hPGRN in NIH3T3 cells. Endogenous hPGRN in HEK 293 cells was also regulated by miR-29b. These findings identify miR-29b as a novel posttranscriptional regulator of PGRN expression, raising the possibility that miR-29b or other miRNAs might be targeted therapeutically to increase hPGRN levels in some FTD patients."xsd:string |
| http://purl.uniprot.org/citations/20479936 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0010551"xsd:string |
| http://purl.uniprot.org/citations/20479936 | http://purl.uniprot.org/core/author | "Jiao J."xsd:string |
| http://purl.uniprot.org/citations/20479936 | http://purl.uniprot.org/core/author | "Farese R.V."xsd:string |
| http://purl.uniprot.org/citations/20479936 | http://purl.uniprot.org/core/author | "Gao F.B."xsd:string |
| http://purl.uniprot.org/citations/20479936 | http://purl.uniprot.org/core/author | "Herl L.D."xsd:string |
| http://purl.uniprot.org/citations/20479936 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20479936 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/20479936 | http://purl.uniprot.org/core/pages | "e10551"xsd:string |
| http://purl.uniprot.org/citations/20479936 | http://purl.uniprot.org/core/title | "MicroRNA-29b regulates the expression level of human progranulin, a secreted glycoprotein implicated in frontotemporal dementia."xsd:string |
| http://purl.uniprot.org/citations/20479936 | http://purl.uniprot.org/core/volume | "5"xsd:string |
| http://purl.uniprot.org/citations/20479936 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20479936 |
| http://purl.uniprot.org/citations/20479936 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20479936 |
| http://purl.uniprot.org/uniprot/P28799#attribution-96D2F7A8D31C227FA22487506FE514A0 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20479936 |