| http://purl.uniprot.org/citations/20483741 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20483741 | http://www.w3.org/2000/01/rdf-schema#comment | "The monomeric GTPase Rap1 controls functional activation of beta2 integrins in leukocytes. In this article, we describe a novel mechanism by which the chemoattractant fMLP activates Rap1 and inside-out signaling of beta2 integrins. We found that fMLP-induced activation of Rap1 in human polymorphonuclear leukocytes or neutrophils and differentiated PLB-985 cells was blocked by inhibitors of the NO/guanosine-3',5'-cyclic monophosphate-dependent protein kinase (cGKI) pathway [N-(3-(aminomethyl)benzyl)acetamidine, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, DT-3 peptide, 8-(4-chlorophenylthio)guanosine 3',5'-cyclic monophosphothioate, Rp-isomer triethylammonium salt-guanosine-3',5'-cyclic monophosphate], indicating that the downstream signaling events in Rap1 activation involve the production of NO and guanosine-3',5'-cyclic monophosphate, as well as the activation of cGKI. Silencing the expression of vasodilator-stimulated phosphoprotein (VASP), a substrate of cGKI, in resting PLB-985 cells or mice neutrophils led to constitutive activation of Rap1. In parallel, silencing VASP in differentiated PLB-985 cells led to recruitment of C3G, a guanine nucleotide exchange factor for Rap1, to the plasma membrane. Expression of murine GFP-tagged phosphodeficient VASP Ser235Ala mutant (murine serine 235 of VASP corresponds to human serine 239) in PLB-985 cells blunted fMLP-induced translocation of C3G to the membrane and activation of Rap1. Thus, bacterial fMLP triggers cGKI-dependent phosphorylation of human VASP on serine 239 and, thereby, controls membrane recruitment of C3G, which is required for activation of Rap1 and beta2 integrin-dependent antibacterial functions of neutrophils."xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.0903910"xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://purl.uniprot.org/core/author | "Walter U."xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://purl.uniprot.org/core/author | "Johnston J.A."xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://purl.uniprot.org/core/author | "Schuh K."xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://purl.uniprot.org/core/author | "Dib K."xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://purl.uniprot.org/core/author | "Kissenpfennig A."xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://purl.uniprot.org/core/author | "Deevi R.K."xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://purl.uniprot.org/core/author | "Koney-Dash M."xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20483741 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://purl.uniprot.org/core/pages | "6575-6584"xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://purl.uniprot.org/core/title | "Vasodilator-stimulated phosphoprotein regulates inside-out signaling of beta2 integrins in neutrophils."xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://purl.uniprot.org/core/volume | "184"xsd:string |
| http://purl.uniprot.org/citations/20483741 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20483741 |
| http://purl.uniprot.org/citations/20483741 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20483741 |
| http://purl.uniprot.org/uniprot/#_P70460-mappedCitation-20483741 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20483741 |
| http://purl.uniprot.org/uniprot/#_P50552-mappedCitation-20483741 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20483741 |
| http://purl.uniprot.org/uniprot/P50552 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20483741 |
| http://purl.uniprot.org/uniprot/P70460 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20483741 |