| http://purl.uniprot.org/citations/20483915 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20483915 | http://www.w3.org/2000/01/rdf-schema#comment | "Non-homologous end-joining (NHEJ), the major repair pathway for DNA double-strand breaks (DSB) in mammalian cells, employs a repertoire of core proteins, the recruitment of which to DSB-ends is Ku-dependent. Lack of either of the core components invariably leads to a repair deficiency. There has been evidence that an alternative end-joining operates in the absence of the core components. We used chromosomal reporter substrates to specifically monitor NHEJ of single I-SceI-induced-DSB for detailed comparison of classical and alternative end-joining. We show that rapid repair of both compatible and non-compatible ends require Ku-protein. In the absence of Ku, cells use a slow but efficient repair mode which experiences increasing sequence-loss with time after DSB induction. Chemical inhibition and PARP1-depletion demonstrated that the alternative end-joining in vivo is completely dependent upon functional PARP1. Furthermore, we show that the requirement for PARP1 depends on the absence of Ku but not on DNA-dependent protein kinase (DNA-PKcs). Extensive sequencing of repair junctions revealed that the alternative rejoining does not require long microhomologies. Together, we show that mammalian cells need Ku for rapid and conservative NHEJ. PARP1-dependent alternative route may partially rescue the deficient repair phenotype presumably at the expense of an enhanced mutation rate."xsd:string |
| http://purl.uniprot.org/citations/20483915 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkq387"xsd:string |
| http://purl.uniprot.org/citations/20483915 | http://purl.uniprot.org/core/author | "Dahm-Daphi J."xsd:string |
| http://purl.uniprot.org/citations/20483915 | http://purl.uniprot.org/core/author | "Mansour W.Y."xsd:string |
| http://purl.uniprot.org/citations/20483915 | http://purl.uniprot.org/core/author | "Rhein T."xsd:string |
| http://purl.uniprot.org/citations/20483915 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20483915 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/20483915 | http://purl.uniprot.org/core/pages | "6065-6077"xsd:string |
| http://purl.uniprot.org/citations/20483915 | http://purl.uniprot.org/core/title | "The alternative end-joining pathway for repair of DNA double-strand breaks requires PARP1 but is not dependent upon microhomologies."xsd:string |
| http://purl.uniprot.org/citations/20483915 | http://purl.uniprot.org/core/volume | "38"xsd:string |
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