| http://purl.uniprot.org/citations/20493910 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20493910 | http://www.w3.org/2000/01/rdf-schema#comment | "Human Apolipoprotein D (apoD) is upregulated under several stress conditions and pathological situations such as neurodegenerative diseases and cancers. We previously showed that apoD mRNA expression is induced in growth-arrested cells and demonstrated the specific binding of nuclear proteins to the region -514 to -475 of the promoter. Such region contains a pair of Serum Responsive Elements (SRE), an Ets-Binding Site (EBS) and a Glucocorticoid Responsive Element (GRE). In this study, we show that Parp-1, HnRNP-U, CBF-A, BUB-3, Kif4, APEX-1 and Ifi204 bind these regulatory elements of the apoD promoter. Specific binding of HnRNP-U and Parp-1 was confirmed by Electrophoretic Mobility Shift Assay (EMSA). In a biotin pull-down assay, Kif4 and BUB-3 bind preferentially the SRE1 and the EBS-GRE sites, respectively, while APEX-1 seems recruited indirectly to these elements. We found that the mRNA expression of some of these binding factors is upregulated in growth-arrested cells and that these proteins also transactivate the apoD promoter. In agreement with these results, mutants of APEX-1 and of Parp-1 defective for their DNA-binding and catalytic activities could not transactivate the promoter. The knockdown of Parp-1 and HnRNP-U and the use of specific inhibitors of MEK1/2 and of Parp-1 also inhibited the induction of apoD gene expression. Moreover, ERK1/2 was found activated in a biphasic manner post serum-starvation and the inhibition of Parp-1 causes a sustained activation of ERK2 but not ERK1 for up to 2h. Altogether, these findings demonstrate the importance of Parp-1, APEX-1 and ERK1/2 catalytic activities in the growth arrest-induced apoD gene expression."xsd:string |
| http://purl.uniprot.org/citations/20493910 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbamcr.2010.04.011"xsd:string |
| http://purl.uniprot.org/citations/20493910 | http://purl.uniprot.org/core/author | "Rassart E."xsd:string |
| http://purl.uniprot.org/citations/20493910 | http://purl.uniprot.org/core/author | "Legault P."xsd:string |
| http://purl.uniprot.org/citations/20493910 | http://purl.uniprot.org/core/author | "Edouard E."xsd:string |
| http://purl.uniprot.org/citations/20493910 | http://purl.uniprot.org/core/author | "Do Carmo S."xsd:string |
| http://purl.uniprot.org/citations/20493910 | http://purl.uniprot.org/core/author | "Levros L.C. Jr."xsd:string |
| http://purl.uniprot.org/citations/20493910 | http://purl.uniprot.org/core/author | "Charfi C."xsd:string |
| http://purl.uniprot.org/citations/20493910 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20493910 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/20493910 | http://purl.uniprot.org/core/pages | "1062-1071"xsd:string |
| http://purl.uniprot.org/citations/20493910 | http://purl.uniprot.org/core/title | "Characterization of nuclear factors modulating the apolipoprotein D promoter during growth arrest: implication of PARP-1, APEX-1 and ERK1/2 catalytic activities."xsd:string |
| http://purl.uniprot.org/citations/20493910 | http://purl.uniprot.org/core/volume | "1803"xsd:string |
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