| http://purl.uniprot.org/citations/20500521 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20500521 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20500521 | http://www.w3.org/2000/01/rdf-schema#comment | "Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic neuropeptide localized in the testis at concentration comparable to that found in the brain, suggesting involvement in spermatogenesis. In this study, we identified the human PACAP testis-specific exon (TSE) 10.9 kb upstream from the translational start site and found that the testis-specific transcript of the human PACAP gene was found to be spliced from the TSE into a region of intron 2 without a frameshift. The resulting PACAP precursor has no signal peptide, suggesting that PACAP functions physiologically in an intracrine manner in the testis. The 5'-flanking region of the TSE contains an 80-bp fragment with potent promoter activity in testicular F9 cell. Electrophoresis mobility shift assays showed that proteins from the F9 nuclear extract interacted specifically with the 80-bp fragment. DNA affinity chromatography allowed isolation of the specific proteins bound to the 80-bp fragment, two of which were identified as Poly (ADP-ribose) polymerase-1 (PARP-1) and TIA-1-related protein (TIAR) by mass spectrometry. By using their siRNAs, the depletion of their proteins in F9 cells affected the potent promoter activity of the 80-bp fragment, suggesting that they might be involved in the testis-specific gene expression of PACAP."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2443.2010.01403.x"xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2443.2010.01403.x"xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Inoue K."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Inoue K."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Sasaki K."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Sasaki K."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Minamino N."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Minamino N."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Handa H."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Handa H."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Miyata A."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Miyata A."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Tominaga A."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Tominaga A."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Hatakeyama M."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Hatakeyama M."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Sugawara H."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Sugawara H."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Futagawa T."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/author | "Futagawa T."xsd:string |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20500521 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |