| http://purl.uniprot.org/citations/20504882 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20504882 | http://www.w3.org/2000/01/rdf-schema#comment | "Nedd4-2, a E3 ubiquitin ligase, regulates epithelial sodium channel-mediated transcellular Na(+) transport in the collecting duct. We investigated the effect of Nedd4-2 on the junctional complex and paracellular conductance in mpkCCD(c14) cells, a collecting duct cell line. We demonstrate that Nedd4-2 coimmunoprecipitated with and reduced the expression of transfected occludin in HEK293 cells. This interaction was mediated via a conserved PY motif in the COOH terminus of occludin and mutation of this PY motif increased the half-life of transfected occludin in HEK293 cells from 6.4 to 11.4 h. We demonstrate that Nedd4-2 ubiquitinates occludin, which was not seen when a catalytically inactive form of Nedd4-2 was used. Overexpression of Nedd4-2 in mpkCCD(c14) cells reduced occludin at the tight junction and transiently increased paracellular conductance in a Ca(2+) switch assay consistent with a delay in the formation of tight junctions. Conversely, siRNA-mediated knockdown of Nedd4-2 increased occludin levels and reduced paracellular conductance. In summary, we demonstrate that Nedd4-2 plays a role in tight junction assembly and the regulation of paracellular conductance in the collecting duct."xsd:string |
| http://purl.uniprot.org/citations/20504882 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajprenal.00674.2009"xsd:string |
| http://purl.uniprot.org/citations/20504882 | http://purl.uniprot.org/core/author | "Thomas C.P."xsd:string |
| http://purl.uniprot.org/citations/20504882 | http://purl.uniprot.org/core/author | "Vandewalle A."xsd:string |
| http://purl.uniprot.org/citations/20504882 | http://purl.uniprot.org/core/author | "Raikwar N.S."xsd:string |
| http://purl.uniprot.org/citations/20504882 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20504882 | http://purl.uniprot.org/core/name | "Am J Physiol Renal Physiol"xsd:string |
| http://purl.uniprot.org/citations/20504882 | http://purl.uniprot.org/core/pages | "F436-44"xsd:string |
| http://purl.uniprot.org/citations/20504882 | http://purl.uniprot.org/core/title | "Nedd4-2 interacts with occludin to inhibit tight junction formation and enhance paracellular conductance in collecting duct epithelia."xsd:string |
| http://purl.uniprot.org/citations/20504882 | http://purl.uniprot.org/core/volume | "299"xsd:string |
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