| http://purl.uniprot.org/citations/20507939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20507939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20507939 | http://www.w3.org/2000/01/rdf-schema#comment | "Phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) catalyzes the hydrolysis of phosphatidylcholine (PC) to generate phosphocholine and diacylglycerol (DAG). PC-PLC has a long tradition in animal signal transduction to generate DAG as a second messenger besides the classical phosphatidylinositol splitting phospholipase C (PI-PLC). Based on amino acid sequence similarity to bacterial PC-PLC, six putative PC-PLC genes (NPC1 to NPC6) were identified in the Arabidopsis genome. RT-PCR analysis revealed overlapping expression pattern of NPC genes in root, stem, leaf, flower, and silique. In auxin-treated P(NPC3):GUS and P(NPC4):GUS seedlings, strong increase of GUS activity was visible in roots, leaves, and shoots and, to a weaker extent, in brassinolide-treated (BL) seedlings. P(NPC4):GUS seedlings also responded to cytokinin with increased GUS activity in young leaves. Compared to wild-type, T-DNA insertional knockouts npc3 and npc4 showed shorter primary roots and lower lateral root density at low BL concentrations but increased lateral root densities in response to exogenous 0.05-1.0 μM BL. BL-induced expression of TCH4 and LRX2, which are involved in cell expansion, was impaired but not impaired in repression of CPD, a BL biosynthesis gene, in BL-treated npc3 and npc4. These observations suggest NPC3 and NPC4 are important in BL-mediated signaling in root growth. When treated with 0.1 μM BL, DAG accumulation was observed in tobacco BY-2 cell cultures labeled with fluorescent PC as early as 15 min after application. We hypothesize that at least one PC-PLC is a plant signaling enzyme in BL signal transduction and, as shown earlier, in elicitor signal transduction."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.org/dc/terms/identifier | "doi:10.1093/mp/ssq005"xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.org/dc/terms/identifier | "doi:10.1093/mp/ssq005"xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/author | "Holk A."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/author | "Holk A."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/author | "Scherer G.F."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/author | "Scherer G.F."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/author | "Wimalasekera R."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/author | "Wimalasekera R."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/author | "Pejchar P."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/author | "Pejchar P."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/author | "Martinec J."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/author | "Martinec J."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/name | "Mol. Plant"xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/name | "Mol. Plant"xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/pages | "610-625"xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/pages | "610-625"xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/title | "Plant phosphatidylcholine-hydrolyzing phospholipases C NPC3 and NPC4 with roles in root development and brassinolide signaling in Arabidopsis thaliana."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/title | "Plant phosphatidylcholine-hydrolyzing phospholipases C NPC3 and NPC4 with roles in root development and brassinolide signaling in Arabidopsis thaliana."xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/volume | "3"xsd:string |
| http://purl.uniprot.org/citations/20507939 | http://purl.uniprot.org/core/volume | "3"xsd:string |