| http://purl.uniprot.org/citations/20516062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20516062 | http://www.w3.org/2000/01/rdf-schema#comment | "The cytokines, interleukin-3 (IL-3), interleukin-5 (IL-5), and granulocyte-macrophage colony-stimulating factor (GM-CSF), exhibit overlapping activities in the regulation of hematopoietic cells. In humans, the common beta (betac) receptor is shared by the three cytokines and functions together with cytokine-specific alpha subunits in signaling. A widely accepted hypothesis is that receptor activation requires heterodisulfide formation between the domain 1 D-E loop disulfide in human betac (hbetac) and unidentified cysteine residues in the N-terminal domains of the alpha receptors. Since the development of this hypothesis, new data have been obtained showing that domain 1 of hbetac is part of the cytokine binding epitope of this receptor and that an IL-3Ralpha isoform lacking the N-terminal Ig-like domain (the "SP2" isoform) is competent for signaling. We therefore investigated whether distortion of the domain 1-domain 4 ligand-binding epitope in hbetac and the related mouse receptor, beta(IL-3), could account for the loss of receptor signaling when the domain 1 D-E loop disulfide is disrupted. Indeed, mutation of the disulfide in hbetac led to both a complete loss of high affinity binding with the human IL-3Ralpha SP2 isoform and of downstream signaling. Mutation of the orthologous residues in the mouse IL-3-specific receptor, beta(IL-3), not only precluded direct binding of mouse IL-3 but also resulted in complete loss of high affinity binding and signaling with the mouse IL-3Ralpha SP2 isoform. Our data are most consistent with a role for the domain 1 D-E loop disulfide of hbetac and beta(IL-3) in maintaining the precise positions of ligand-binding residues necessary for normal high affinity binding and signaling."xsd:string |
| http://purl.uniprot.org/citations/20516062 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.097881"xsd:string |
| http://purl.uniprot.org/citations/20516062 | http://purl.uniprot.org/core/author | "Chen J."xsd:string |
| http://purl.uniprot.org/citations/20516062 | http://purl.uniprot.org/core/author | "Young I.G."xsd:string |
| http://purl.uniprot.org/citations/20516062 | http://purl.uniprot.org/core/author | "Murphy J.M."xsd:string |
| http://purl.uniprot.org/citations/20516062 | http://purl.uniprot.org/core/author | "Mirza S."xsd:string |
| http://purl.uniprot.org/citations/20516062 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20516062 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/20516062 | http://purl.uniprot.org/core/pages | "24759-24768"xsd:string |
| http://purl.uniprot.org/citations/20516062 | http://purl.uniprot.org/core/title | "The role of interchain heterodisulfide formation in activation of the human common beta and mouse betaIL-3 receptors."xsd:string |
| http://purl.uniprot.org/citations/20516062 | http://purl.uniprot.org/core/volume | "285"xsd:string |
| http://purl.uniprot.org/citations/20516062 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20516062 |
| http://purl.uniprot.org/citations/20516062 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20516062 |
| http://purl.uniprot.org/uniprot/#_Q0PMN2-mappedCitation-20516062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20516062 |
| http://purl.uniprot.org/uniprot/#_A0A2R8VHE8-mappedCitation-20516062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20516062 |
| http://purl.uniprot.org/uniprot/#_B4DZL8-mappedCitation-20516062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20516062 |
| http://purl.uniprot.org/uniprot/#_Q3U241-mappedCitation-20516062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20516062 |
| http://purl.uniprot.org/uniprot/#_P01586-mappedCitation-20516062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20516062 |
| http://purl.uniprot.org/uniprot/#_P08700-mappedCitation-20516062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20516062 |
| http://purl.uniprot.org/uniprot/#_P26954-mappedCitation-20516062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20516062 |
| http://purl.uniprot.org/uniprot/#_P32927-mappedCitation-20516062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20516062 |
| http://purl.uniprot.org/uniprot/#_Q3U1W0-mappedCitation-20516062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20516062 |
| http://purl.uniprot.org/uniprot/#_Q3U1Z1-mappedCitation-20516062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20516062 |
| http://purl.uniprot.org/uniprot/#_Q3U3P4-mappedCitation-20516062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20516062 |