| http://purl.uniprot.org/citations/20524965 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20524965 | http://www.w3.org/2000/01/rdf-schema#comment | "Neuroendocrine regulatory peptide (NERP)-1 and NERP-2 are biologically active peptides recently discovered by peptidomic analysis. NERPs are processed out from the 594-residue VGF protein which contains many prohormone convertase cleavage motifs. VGF-deficient mice exhibit a hypermetabolic and infertile phenotype, for which VGF protein-derived peptides including NERPs are presumably responsible. To provide a solid basis for elucidating physiological roles of NERPs, we investigated rat VGF protein processing by chromatographic and mass spectrometric analysis, and immunoblotting, using antibodies against NERPs and the VGF protein C-terminus (VGF-C). Cellular and tissue distribution of immunoreactive (ir) NERPs were also analyzed in the rat. Both ir-NERP-1 and ir-NERP-2, which occur abundantly in the CNS and pituitary, moderately in the gastrointestinal (GI) tract, were mainly localized in neuronal structures. Major endogenous forms of ir-NERPs in the brain and GI tract were identified as NERP-1, NERP-2, and big NERP-2 (NERP-1 + NERP-2), with NERP-1 and big NERP-2 being predominant. Regarding ir-VGF-C peptides, VGF[588-617], VGF[556-617], and VGF[509-617] were found to be major forms. Immunoblotting with the NERP-2 and VGF-C antibodies revealed processing intermediates of 10-37 kDa. Taken together, we deduce that VGF protein is primarily cleaved at 10 sites through the processing pathway common to the brain and GI tract."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1471-4159.2010.06827.x"xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Yamaguchi H."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Matsuo T."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Shioda S."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Takao T."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Sasaki K."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Minamino N."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Satomi Y."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Kageyama H."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Matsubara M."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Yoshizawa-Kumagaye K."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Ishizu T."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Nakazato M."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Date Y."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/author | "Mishiro-Sato E."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/name | "J Neurochem"xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/pages | "1097-1106"xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/title | "Distribution of neuroendocrine regulatory peptide-1 and -2, and proteolytic processing of their precursor VGF protein in the rat."xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://purl.uniprot.org/core/volume | "114"xsd:string |
| http://purl.uniprot.org/citations/20524965 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20524965 |
| http://purl.uniprot.org/citations/20524965 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20524965 |
| http://purl.uniprot.org/uniprot/#_A6J052-mappedCitation-20524965 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20524965 |