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http://purl.uniprot.org/citations/20543037http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20543037http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20543037http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20543037http://www.w3.org/2000/01/rdf-schema#comment"Most gene knockouts in mycoplasmas are achieved through labor-intensive transposon mutagenesis. Here, we describe a method for making targeted deletions in Mycoplasma pneumoniae by use of homologous recombination. In this method, M. pneumoniae is transformed with a plasmid carrying an antibiotic resistance marker flanked by 1-kb regions surrounding the target gene. Following selection for the antibiotic resistance, colonies are screened for double crossovers which indicate complete deletion of the target open reading frame."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.org/dc/terms/identifier"doi:10.1128/aem.00024-10"xsd:string
http://purl.uniprot.org/citations/20543037http://purl.org/dc/terms/identifier"doi:10.1128/aem.00024-10"xsd:string
http://purl.uniprot.org/citations/20543037http://purl.org/dc/terms/identifier"doi:10.1128/aem.00024-10"xsd:string
http://purl.uniprot.org/citations/20543037http://purl.org/dc/terms/identifier"doi:10.1128/AEM.00024-10"xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Glass J.I."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Glass J.I."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Glass J.I."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Assad-Garcia N."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Assad-Garcia N."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Assad-Garcia N."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Benders G.A."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Benders G.A."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Benders G.A."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Krishnakumar R."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Krishnakumar R."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Krishnakumar R."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Montague M.G."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Montague M.G."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Montague M.G."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Phan Q."xsd:string
http://purl.uniprot.org/citations/20543037http://purl.uniprot.org/core/author"Phan Q."xsd:string