| http://purl.uniprot.org/citations/20544854 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20544854 | http://www.w3.org/2000/01/rdf-schema#comment | "Cellular zinc plays a key role in lysosomal change and cell death in neurons and astrocytes under oxidative stress. Here, using astrocytes lacking metallothionein-3 (MT3), a potential source of labile zinc in the brain, we studied the role of MT3 in oxidative stress responses. H(2)O(2) induced a large increase in labile zinc in wild-type (WT) astrocytes, but stimulated only a modest rise in MT3-null astrocytes. In addition, H(2)O(2)-induced lysosomal membrane permeabilization (LMP) and cell death were comparably attenuated in MT3-null astrocytes. Expression and glycosylation of Lamp1 (lysosome-associated membrane protein 1) and Lamp2 were increased in MT3-null astrocytes, and the activities of several lysosomal enzymes were significantly reduced, indicating an effect of MT3 on lysosomal components. Consistent with lysosomal dysfunction in MT3-null cells, the level of LC3-II (microtubule-associated protein 1 light chain 3), a marker of early autophagy, was increased by oxidative stress in WT astrocytes, but not in MT3-null cells. Similar changes in Lamp1, LC3, and cathepsin-D were induced by the lysosomal inhibitors bafilomycin A1, chloroquine, and monensin, indicating that lysosomal dysfunction may lie upstream of changes observed in MT3-null astrocytes. Consistent with this idea, lysosomal accumulation of cholesterol and lipofuscin were augmented in MT3-null astrocytes. Similar to the results seen in MT3-null cells, MT3 knockdown by siRNA inhibited oxidative stress-induced increases in zinc and LMP. These results indicate that MT3 may play a key role in normal lysosomal function in cultured astrocytes."xsd:string |
| http://purl.uniprot.org/citations/20544854 | http://purl.org/dc/terms/identifier | "doi:10.1002/glia.20998"xsd:string |
| http://purl.uniprot.org/citations/20544854 | http://purl.uniprot.org/core/author | "Kim H.J."xsd:string |
| http://purl.uniprot.org/citations/20544854 | http://purl.uniprot.org/core/author | "Lee S.J."xsd:string |
| http://purl.uniprot.org/citations/20544854 | http://purl.uniprot.org/core/author | "Park M.H."xsd:string |
| http://purl.uniprot.org/citations/20544854 | http://purl.uniprot.org/core/author | "Koh J.Y."xsd:string |
| http://purl.uniprot.org/citations/20544854 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20544854 | http://purl.uniprot.org/core/name | "Glia"xsd:string |
| http://purl.uniprot.org/citations/20544854 | http://purl.uniprot.org/core/pages | "1186-1196"xsd:string |
| http://purl.uniprot.org/citations/20544854 | http://purl.uniprot.org/core/title | "Metallothionein-3 regulates lysosomal function in cultured astrocytes under both normal and oxidative conditions."xsd:string |
| http://purl.uniprot.org/citations/20544854 | http://purl.uniprot.org/core/volume | "58"xsd:string |
| http://purl.uniprot.org/citations/20544854 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20544854 |
| http://purl.uniprot.org/citations/20544854 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20544854 |
| http://purl.uniprot.org/uniprot/P28184#attribution-135701E641694F0A772E6A10A6AEB96A | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20544854 |
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| http://purl.uniprot.org/uniprot/#_P28184-mappedCitation-20544854 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20544854 |
| http://purl.uniprot.org/uniprot/#_Q3USP9-mappedCitation-20544854 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20544854 |
| http://purl.uniprot.org/uniprot/#_P02798-mappedCitation-20544854 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20544854 |
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| http://purl.uniprot.org/uniprot/#_Q3U340-mappedCitation-20544854 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20544854 |