| http://purl.uniprot.org/citations/20558763 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20558763 | http://www.w3.org/2000/01/rdf-schema#comment | "The contraction of gallbladders (GBs) with cholesterol stones is impaired due to high cholesterol concentrations in caveolae compared with GBs with pigment stones. The reduced contraction is caused by a lower cholecystokinin (CCK)-8 binding to CCK-1 receptors (CCK-1R) due to caveolar sequestration of receptors. We aimed to examine the mechanism of cholesterol-induced sequestration of receptors. Muscle cells from human and guinea pig GBs were studied. Antibodies were used to examine CCK-1R, antigens of early and recycling endosomes, and total (CAV-3) and phosphorylated caveolar-3 protein (pCAV-3) by Western blots. Contraction was measured in muscle cells transfected with CAV3 mRNA or clathrin heavy-chain small-interfering RNA (siRNA). CCK-1R returned back to the bulk plasma membrane (PM) 30 min after CCK-8 recycled by endosomes, peaking at 5 min in early endosomes and at 20 min in recycling endosomes. Pretreatment with cholesterol-rich liposomes inhibited the transfer of CCK-1R and of CAV-3 in the endosomes by blocking CAV-3 phosphorylation. 4-Amino-5-(4-chloro-phenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (inhibitor of tyrosine kinase) reproduced these effects by blocking pCAV-3 formation, increasing CAV-3 and CCK-1R sequestration in the caveolae and impairing CCK-8-induced contraction. CAV-3 siRNA reduced CAV-3 protein expression, decreased CCK-8-induced contraction, and accumulated CCK-1R in the caveolae. Abnormal concentrations of caveolar cholesterol had no effect on met-enkephalin that stimulates a delta-opioid receptor that internalizes through clathrin. We found that impaired muscle contraction in GBs with cholesterol stones is due to high caveolar levels of cholesterol that inhibits pCAV-3 generation. Caveolar cholesterol increases the caveolar sequestration of CAV-3 and CCK-1R caused by their reduced recycling to the PM."xsd:string |
| http://purl.uniprot.org/citations/20558763 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpgi.00064.2010"xsd:string |
| http://purl.uniprot.org/citations/20558763 | http://purl.uniprot.org/core/author | "Cong P."xsd:string |
| http://purl.uniprot.org/citations/20558763 | http://purl.uniprot.org/core/author | "Behar J."xsd:string |
| http://purl.uniprot.org/citations/20558763 | http://purl.uniprot.org/core/author | "Biancani P."xsd:string |
| http://purl.uniprot.org/citations/20558763 | http://purl.uniprot.org/core/author | "Pricolo V."xsd:string |
| http://purl.uniprot.org/citations/20558763 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20558763 | http://purl.uniprot.org/core/name | "Am J Physiol Gastrointest Liver Physiol"xsd:string |
| http://purl.uniprot.org/citations/20558763 | http://purl.uniprot.org/core/pages | "G742-50"xsd:string |
| http://purl.uniprot.org/citations/20558763 | http://purl.uniprot.org/core/title | "Effects of cholesterol on CCK-1 receptors and caveolin-3 proteins recycling in human gallbladder muscle."xsd:string |
| http://purl.uniprot.org/citations/20558763 | http://purl.uniprot.org/core/volume | "299"xsd:string |
| http://purl.uniprot.org/citations/20558763 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20558763 |
| http://purl.uniprot.org/citations/20558763 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20558763 |
| http://purl.uniprot.org/uniprot/#_P32238-mappedCitation-20558763 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20558763 |
| http://purl.uniprot.org/uniprot/P32238 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20558763 |