| http://purl.uniprot.org/citations/20581823 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20581823 | http://www.w3.org/2000/01/rdf-schema#comment | "RIG-I is a cytosolic helicase that senses 5'-ppp RNA contained in negative-strand RNA viruses and triggers innate antiviral immune responses. Calorimetric binding studies established that the RIG-I C-terminal regulatory domain (CTD) binds to blunt-end double-stranded 5'-ppp RNA a factor of 17 more tightly than to its single-stranded counterpart. Here we report on the crystal structure of RIG-I CTD bound to both blunt ends of a self-complementary 5'-ppp dsRNA 12-mer, with interactions involving 5'-pp clearly visible in the complex. The structure, supported by mutation studies, defines how a lysine-rich basic cleft within the RIG-I CTD sequesters the observable 5'-pp of the bound RNA, with a stacked phenylalanine capping the terminal base pair. Key intermolecular interactions observed in the crystalline state are retained in the complex of 5'-ppp dsRNA 24-mer and full-length RIG-I under in vivo conditions, as evaluated from the impact of binding pocket RIG-I mutations and 2'-OCH(3) RNA modifications on the interferon response."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.org/dc/terms/identifier | "doi:10.1038/nsmb.1863"xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/author | "Li H."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/author | "Sheng G."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/author | "Patel D.J."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/author | "Tuschl T."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/author | "Juranek S."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/author | "Schuberth C."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/author | "Hartmann G."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/author | "Schlee M."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/author | "Micura R."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/author | "Goldeck M."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/author | "Ludwig J."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/name | "Nat Struct Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/pages | "781-787"xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/title | "Structural and functional insights into 5'-ppp RNA pattern recognition by the innate immune receptor RIG-I."xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://purl.uniprot.org/core/volume | "17"xsd:string |
| http://purl.uniprot.org/citations/20581823 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20581823 |
| http://purl.uniprot.org/citations/20581823 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20581823 |
| http://purl.uniprot.org/uniprot/O95786#attribution-D3202F3A658849A5C3B6A7D1148D5DBF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20581823 |
| http://purl.uniprot.org/uniprot/#_B3KN51-mappedCitation-20581823 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20581823 |
| http://purl.uniprot.org/uniprot/#_B4DWT9-mappedCitation-20581823 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20581823 |