Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/20588169http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20588169http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20588169http://www.w3.org/2000/01/rdf-schema#comment"

Objective

The innate immune component TRIM5alpha has the ability to restrict retrovirus infection in a species-specific manner. TRIM5alpha of some primate species restricts infection by HIV-1, whereas human TRIM5alpha lacks this specificity. Previous studies have suggested that certain polymorphisms in human TRIM5alpha may enhance or impair the proteins affinity for HIV-1. This study investigates the role of TRIM5alpha polymorphisms in resistance/susceptibility to HIV-1 within the Pumwani sex worker cohort in Nairobi, Kenya. A group of women within this cohort remain HIV-1-seronegative and PCR-negative despite repeated exposure to HIV-1 through active sex work.

Design

A 1 kb fragment of the TRIM5alpha gene, including exon 2, from 1032 women enrolled in the Pumwani sex worker cohort was amplified and sequenced. Single-nucleotide polymorphisms (SNPs) and haplotypes were compared between HIV-1-positive and resistant women.

Methods

The TRIM5alpha exon 2 genomic fragment was amplified, sequenced and genotyped. Pypop32-0.6.0 was used to determine SNP and haplotype frequencies and statistical analysis was carried out using SPSS-13.0 for Windows.

Results

A TRIM5alpha SNP (rs10838525) resulting in the amino acid change from arginine to glutamine at codon 136, was enriched in HIV-1-resistant individuals [P = 1.104E-05; odds ratio (OR) 2.991; 95% confidence interval (CI) 1.806-4.953] and women with 136Q were less likely to seroconvert (P = 0.002; log-rank 12.799). Wild-type TRIM5alpha exon 2 was associated with susceptibility to HIV-1 (P = 0.006; OR 0.279; 95% CI 0.105-0.740) and rapid seroconversion (P = 0.001; log-rank 14.475).

Conclusions

Our findings suggest that a shift from arginine to glutamine at codon 136 in the coiled-coil region of TRIM5alpha confers protection against HIV-1 in the Pumwani sex worker cohort."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.org/dc/terms/identifier"doi:10.1097/QAD.0b013e32833b5256"xsd:string
http://purl.uniprot.org/citations/20588169http://purl.org/dc/terms/identifier"doi:10.1097/qad.0b013e32833b5256"xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Luo M."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Luo M."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Plummer F.A."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Plummer F.A."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Price H."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Price H."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Ball T.B."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Ball T.B."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Kimani J."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Kimani J."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Lacap P."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Lacap P."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Tuff J."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Tuff J."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Wachihi C."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/author"Wachihi C."xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/name"AIDS"xsd:string
http://purl.uniprot.org/citations/20588169http://purl.uniprot.org/core/name"AIDS"xsd:string
▶