| http://purl.uniprot.org/citations/20603002 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20603002 | http://www.w3.org/2000/01/rdf-schema#comment | "The AP2 adaptor complex (alpha, beta2, sigma2, and mu2 subunits) crosslinks the endocytic clathrin scaffold to PtdIns4,5P(2)-containing membranes and transmembrane protein cargo. In the "locked" cytosolic form, AP2's binding sites for the two endocytic motifs, YxxPhi on the C-terminal domain of mu2 (C-mu2) and [ED]xxxL[LI] on sigma2, are blocked by parts of beta2. Using protein crystallography, we show that AP2 undergoes a large conformational change in which C-mu2 relocates to an orthogonal face of the complex, simultaneously unblocking both cargo-binding sites; the previously unstructured mu2 linker becomes helical and binds back onto the complex. This structural rearrangement results in AP2's four PtdIns4,5P(2)- and two endocytic motif-binding sites becoming coplanar, facilitating their simultaneous interaction with PtdIns4,5P(2)/cargo-containing membranes. Using a range of biophysical techniques, we show that the endocytic cargo binding of AP2 is driven by its interaction with PtdIns4,5P(2)-containing membranes."xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cell.2010.05.006"xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/author | "McCoy A.J."xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/author | "Honing S."xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/author | "Collins B.M."xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/author | "Evans P.R."xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/author | "Owen D.J."xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/author | "Jackson L.P."xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/author | "Kelly B.T."xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/author | "James L.C."xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/author | "Gaffry T."xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/name | "Cell"xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/pages | "1220-1229"xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/title | "A large-scale conformational change couples membrane recruitment to cargo binding in the AP2 clathrin adaptor complex."xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://purl.uniprot.org/core/volume | "141"xsd:string |
| http://purl.uniprot.org/citations/20603002 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20603002 |
| http://purl.uniprot.org/citations/20603002 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20603002 |
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| http://purl.uniprot.org/uniprot/#_O43493-mappedCitation-20603002 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20603002 |