| http://purl.uniprot.org/citations/20610383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20610383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20610383 | http://www.w3.org/2000/01/rdf-schema#comment | "Talin is an adaptor protein that couples integrins to F-actin. Structural studies show that the N-terminal talin head contains an atypical FERM domain, whereas the N- and C-terminal parts of the talin rod include a series of α-helical bundles. However, determining the structure of the central part of the rod has proved problematic. Residues 1359-1659 are homologous to the MESDc1 gene product, and we therefore expressed this region of talin in Escherichia coli. The crystal structure shows a unique fold comprised of a 5- and 4-helix bundle. The 5-helix bundle is composed of nonsequential helices due to insertion of the 4-helix bundle into the loop at the C terminus of helix α3. The linker connecting the bundles forms a two-stranded anti-parallel β-sheet likely limiting the relative movement of the two bundles. Because the 5-helix bundle contains the N and C termini of this module, we propose that it is linked by short loops to adjacent bundles, whereas the 4-helix bundle protrudes from the rod. This suggests the 4-helix bundle has a unique role, and its pI (7.8) is higher than other rod domains. Both helical bundles contain vinculin-binding sites but that in the isolated 5-helix bundle is cryptic, whereas that in the isolated 4-helix bundle is constitutively active. In contrast, both bundles are required for actin binding. Finally, we show that the MESDc1 protein, which is predicted to have a similar fold, is a novel actin-binding protein."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.095455"xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m109.095455"xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Patel B."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Patel B."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Barsukov I.L."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Barsukov I.L."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Bate N."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Bate N."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Critchley D.R."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Critchley D.R."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Gingras A.R."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Gingras A.R."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Goult B.T."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Goult B.T."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Roberts G.C."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Roberts G.C."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Emsley J."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Emsley J."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Kopp P.M."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/author | "Kopp P.M."xsd:string |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20610383 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |