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http://purl.uniprot.org/citations/20622165http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20622165http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Pertussis toxin uncoupling-based studies have shown that Gαi and Gαo can inhibit insulin secretion in pancreatic β-cells. Yet it is unclear whether Gαi and Gαo operate through identical mechanisms and how these G-protein-mediated signals inhibit insulin secretion in vivo. Our objective is to examine whether/how Gαo regulates islet development and insulin secretion in β-cells.

Research design and methods

Immunoassays were used to analyze the Gαo expression in mouse pancreatic cells. Gαo was specifically inactivated in pancreatic progenitor cells by pancreatic cell-specific gene deletion. Hormone expression and insulin secretion in response to different stimuli were assayed in vivo and in vitro. Electron microscope and total internal reflection fluorescence-based assays were used to evaluate how Gαo regulates insulin vesicle docking and secretion in response to glucose stimulation.

Results

Islet cells differentiate properly in Gαo(-/-) mutant mice. Gαo inactivation significantly enhances insulin secretion both in vivo and in isolation. Gαo nullizygous β-cells contain an increased number of insulin granules docked on the cell plasma membrane, although the total number of vesicles per β-cell remains unchanged.

Conclusions

Gαo is not required for endocrine islet cell differentiation, but it regulates the number of insulin vesicles docked on the β-cell membrane."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.org/dc/terms/identifier"doi:10.2337/db09-1719"xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Hao Y."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Xu Y."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Zhao A."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Birnbaumer L."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Jiang M."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Abramowitz J."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Gu G."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Nagamatsu S."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Ohara-Imaizumi M."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Boulay G."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Powers A.C."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Piston D."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Benninger R.K."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/author"Brissova M."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/name"Diabetes"xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/pages"2522-2529"xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/title"Galphao represses insulin secretion by reducing vesicular docking in pancreatic beta-cells."xsd:string
http://purl.uniprot.org/citations/20622165http://purl.uniprot.org/core/volume"59"xsd:string
http://purl.uniprot.org/citations/20622165http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20622165
http://purl.uniprot.org/citations/20622165http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/20622165
http://purl.uniprot.org/uniprot/P18872#attribution-626BBA87F610B4F592368480C7501C27http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/20622165
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