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http://purl.uniprot.org/citations/20624875http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20624875http://www.w3.org/2000/01/rdf-schema#comment"c-Ski has been known to be phosphorylated at serine residue(s), which results in slower migration of c-Ski in SDS-polyacrylamide gel electrophoresis. The position(s) of phosphorylation, however, has not been determined. In the present study, we identified a phosphorylation site of c-Ski which affects its electrophoretic motility as serine 515 using MALDI-TOF mass spectrometry. A phosphorylation-resistant mutant, c-Ski S515A, did not exhibit a phosphatase-sensitive band shift. In addition, we confirmed that endogenous c-Ski is phosphorylated at serine 515, using a specific antibody. The phosphorylation status of c-Ski, however, does not appear to affect its stability or effects on TGF-β signalling. Identification of the phosphorylation site of c-Ski would allow us further examination of physiological significance of c-Ski phosphorylation."xsd:string
http://purl.uniprot.org/citations/20624875http://purl.org/dc/terms/identifier"doi:10.1093/jb/mvq076"xsd:string
http://purl.uniprot.org/citations/20624875http://purl.uniprot.org/core/author"Nagata S."xsd:string
http://purl.uniprot.org/citations/20624875http://purl.uniprot.org/core/author"Miyazono K."xsd:string
http://purl.uniprot.org/citations/20624875http://purl.uniprot.org/core/author"Nagata M."xsd:string
http://purl.uniprot.org/citations/20624875http://purl.uniprot.org/core/author"Saitoh M."xsd:string
http://purl.uniprot.org/citations/20624875http://purl.uniprot.org/core/author"Yuki K."xsd:string
http://purl.uniprot.org/citations/20624875http://purl.uniprot.org/core/author"Miyazawa K."xsd:string
http://purl.uniprot.org/citations/20624875http://purl.uniprot.org/core/author"Isogaya K."xsd:string
http://purl.uniprot.org/citations/20624875http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20624875http://purl.uniprot.org/core/name"J Biochem"xsd:string
http://purl.uniprot.org/citations/20624875http://purl.uniprot.org/core/pages"423-427"xsd:string
http://purl.uniprot.org/citations/20624875http://purl.uniprot.org/core/title"Identification of a phosphorylation site in c-Ski as serine 515."xsd:string
http://purl.uniprot.org/citations/20624875http://purl.uniprot.org/core/volume"148"xsd:string
http://purl.uniprot.org/citations/20624875http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20624875
http://purl.uniprot.org/citations/20624875http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/20624875
http://purl.uniprot.org/uniprot/#_P12755-mappedCitation-20624875http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20624875
http://purl.uniprot.org/uniprot/#_Q7Z462-mappedCitation-20624875http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20624875
http://purl.uniprot.org/uniprot/P12755http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/20624875
http://purl.uniprot.org/uniprot/Q7Z462http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/20624875