| http://purl.uniprot.org/citations/20655338 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20655338 | http://www.w3.org/2000/01/rdf-schema#comment | "Carboxypeptidase E (CPE) is an exopeptidase that removes C-terminal basic amino acids from a variety of bioactive peptides. In addition to this role, data obtained in recent years has supported a potential function for CPE as a sorting receptor, helping direct peptides destined for regulated secretion from the trans-Golgi to granules in preparation for release. This possible sorting function was assessed using mouse AtT-20 cells, a well-established corticotroph cell line that synthesizes and releases POMC/ACTH in regulated fashion. Cells that were treated with siRNA to Cpe effectively suppressed CPE expression. ACTH was released in a regulated fashion from CPE-depleted cells in response to two secretagogues, 8-bromo-cyclic AMP and corticotrophin-releasing hormone. POMC/ACTH content of CPE-depleted cells was higher than that of control cells, but both released a similar percentage of ACTH content in response to secretagogue addition. Cells depleted of CPE generally secreted more high-molecular weight forms of POMC/ACTH under basal conditions than control cells; however, the CPE-depleted cells responded to a secretagogue by releasing newly synthesized ACTH 1-39 in a manner similar to controls. These results, whereby RNAi was used to acutely suppress CPE, do not support a role for this protein as necessary for or central to sorting of POMC/ACTH to the regulated secretory pathway in AtT-20 cells."xsd:string |
| http://purl.uniprot.org/citations/20655338 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.regpep.2010.07.162"xsd:string |
| http://purl.uniprot.org/citations/20655338 | http://purl.uniprot.org/core/author | "Behrend E.N."xsd:string |
| http://purl.uniprot.org/citations/20655338 | http://purl.uniprot.org/core/author | "Kemppainen R.J."xsd:string |
| http://purl.uniprot.org/citations/20655338 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20655338 | http://purl.uniprot.org/core/name | "Regul Pept"xsd:string |
| http://purl.uniprot.org/citations/20655338 | http://purl.uniprot.org/core/pages | "174-179"xsd:string |
| http://purl.uniprot.org/citations/20655338 | http://purl.uniprot.org/core/title | "Acute inhibition of carboxypeptidase E expression in AtT-20 cells does not affect regulated secretion of ACTH."xsd:string |
| http://purl.uniprot.org/citations/20655338 | http://purl.uniprot.org/core/volume | "165"xsd:string |
| http://purl.uniprot.org/citations/20655338 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20655338 |
| http://purl.uniprot.org/citations/20655338 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20655338 |
| http://purl.uniprot.org/uniprot/#_Q00493-mappedCitation-20655338 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20655338 |
| http://purl.uniprot.org/uniprot/#_Q543R4-mappedCitation-20655338 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20655338 |
| http://purl.uniprot.org/uniprot/Q00493 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20655338 |
| http://purl.uniprot.org/uniprot/Q543R4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20655338 |