| http://purl.uniprot.org/citations/2066339 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2066339 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2066339 | http://www.w3.org/2000/01/rdf-schema#comment | "The narL gene product, NarL, is the nitrate-responsive regulator of anaerobic respiratory gene expression. We used genetic analysis of narL mutants to better understand the mechanism of NarL-mediated gene regulation. We selected and analyzed seven nitrate-independent narL mutants. Each of three independent, strongly constitutive mutants had changes of Val-88 to Ala. The other four mutants were weakly constitutive. The narL505(V88A) allele was largely dominant to narL+, while narX+ had a negative influence on its constitutive phenotype, suggesting that NarX may play a negative role in nitrate regulation. We also constructed two narL mutations that are analogous to previously characterized constitutive degU alleles. The first, narL503(H15L), was a recessive null allele. The second, narL504(D110K), functioned essentially as wild type but was dependent on narX+ for full activity. We changed Asp-59 of NarL, which corresponds to the site of phosphorylation of other response regulators, to Asn. This change, narL502(D59N), was a recessive null allele, which is consistent with the hypothesis that NarL requires phosphorylation for activation. Finally, we tested the requirement for molybdate on regulation in a narL505(V88A) strain. Although narL505(V88A) conferred some nitrate-independent expression of fdnGHI (encoding formate dehydrogenase-N) in limiting molybdate, it required excess molybdate for full induction both in the absence and in the presence of nitrate. This finding suggests that narL505(V88A) did not confer molybdate-independent expression of fdnGHI."xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.173.14.4424-4432.1991"xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.173.14.4424-4432.1991"xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/author | "Stewart V."xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/author | "Stewart V."xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/author | "Egan S.M."xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/author | "Egan S.M."xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/pages | "4424-4432"xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/pages | "4424-4432"xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/title | "Mutational analysis of nitrate regulatory gene narL in Escherichia coli K-12."xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/title | "Mutational analysis of nitrate regulatory gene narL in Escherichia coli K-12."xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/volume | "173"xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://purl.uniprot.org/core/volume | "173"xsd:string |
| http://purl.uniprot.org/citations/2066339 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2066339 |
| http://purl.uniprot.org/citations/2066339 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2066339 |
| http://purl.uniprot.org/citations/2066339 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2066339 |
| http://purl.uniprot.org/citations/2066339 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2066339 |
| http://purl.uniprot.org/uniprot/P0AF28 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/2066339 |
| http://purl.uniprot.org/uniprot/#_P0AF28-citation-2066339 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/2066339 |