| http://purl.uniprot.org/citations/20668219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20668219 | http://www.w3.org/2000/01/rdf-schema#comment | "Signaling lymphocytic activation molecule-associated protein (SAP), an adaptor molecule that recruits Fyn to the signaling lymphocytic activation molecule (SLAM) family of immunomodulatory receptors, is mutated in X-linked lymphoproliferative disease. CD4(+) T cells from SAP-deficient mice have defective TCR-induced and follicular Th cell IL-4 production and impaired T cell-mediated help for germinal center formation; however, the downstream intermediates contributing to these defects remain unclear. We previously found that SAP-deficient CD4(+) T cells exhibit decreased protein kinase C (PKC)-theta recruitment upon TCR stimulation. We demonstrate in this paper using GST pulldowns and coimmunoprecipitation studies that SAP constitutively associates with PKC-in T cells. SAP-PKC-theta interactions required R78 of SAP, a residue previously implicated in Fyn recruitment, yet SAP's interactions with PKC-theta occurred independent of phosphotyrosine binding and Fyn. Overexpression of SAP in T cells increased and sustained PKC-theta recruitment to the immune synapse and elevated IL-4 production in response to TCR plus SLAM-mediated stimulation. Moreover, PKC-theta, like SAP, was required for SLAM-mediated increases in IL-4 production, and, conversely, membrane-targeted PKC-theta mutants rescued IL-4 expression in SAP(-/-) CD4(+) T cells, providing genetic evidence that PKC-theta is a critical component of SLAM/SAP-mediated pathways that influence TCR-driven IL-4 production."xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.0902182"xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/author | "Dong B."xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/author | "Wu J.Z."xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/author | "Siminovitch K.A."xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/author | "Schwartzberg P.L."xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/author | "Cannons J.L."xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/author | "Shaw S."xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/author | "Gomez-Rodriguez J."xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/pages | "2819-2827"xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/title | "Biochemical and genetic evidence for a SAP-PKC-theta interaction contributing to IL-4 regulation."xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://purl.uniprot.org/core/volume | "185"xsd:string |
| http://purl.uniprot.org/citations/20668219 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20668219 |
| http://purl.uniprot.org/citations/20668219 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20668219 |
| http://purl.uniprot.org/uniprot/#_D3YVZ0-mappedCitation-20668219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20668219 |
| http://purl.uniprot.org/uniprot/#_D3YZ57-mappedCitation-20668219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20668219 |
| http://purl.uniprot.org/uniprot/#_B2RWI0-mappedCitation-20668219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20668219 |
| http://purl.uniprot.org/uniprot/#_A6H667-mappedCitation-20668219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20668219 |
| http://purl.uniprot.org/uniprot/#_D3YZA2-mappedCitation-20668219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20668219 |
| http://purl.uniprot.org/uniprot/#_F8WGT6-mappedCitation-20668219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20668219 |