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| http://purl.uniprot.org/citations/20669324 | http://www.w3.org/2000/01/rdf-schema#comment | "Synapse malformation underlies numerous neurodevelopmental illnesses, including autism spectrum disorders. Here we identify the lipid raft protein flotillin-1 as a promoter of glutamatergic synapse formation. We cultured neurons from the hippocampus, a brain region important for learning and memory, and examined them at two weeks in vitro, a time period rich with synapse formation. Double-label immunocytochemistry of native flot-1 with glutamatergic and GABAergic synapse markers showed that flot-1 was preferentially colocalized with the glutamatergic presynaptic marker vesicular glutamate transporter 1 (VGLUT1), compared to the GABAergic presynaptic marker glutamic acid decarboxylase-65 (GAD-65). Triple-label immunocytochemistry of native flot-1, VGLUT1, and NR1, the obligatory subunit of NMDA receptors, indicates that Flot-1 was preferentially localized to synaptic rather than extrasynaptic NR1. Furthermore, electrophysiological results using whole-cell patch clamp showed that Flot-1 increased the frequency of miniature excitatory postsynaptic currents (mEPSCs) but not miniature inhibitory postsynaptic currents (mIPSCs), whereas amplitude and decay kinetics of either type of synaptic current was not affected. Corresponding immunocytochemical data confirmed that the number of glutamatergic synapses increased with flot-1 overexpression. Overall, our anatomical and physiological results show that flot-1 enhances the formation of glutamatergic synapses but not GABAergic synapses, suggesting that the role of flot-1 in neurodevelopmental disorders should be explored."xsd:string |
| http://purl.uniprot.org/citations/20669324 | http://purl.org/dc/terms/identifier | "doi:10.1002/dneu.20828"xsd:string |
| http://purl.uniprot.org/citations/20669324 | http://purl.uniprot.org/core/author | "Wenthold R.J."xsd:string |
| http://purl.uniprot.org/citations/20669324 | http://purl.uniprot.org/core/author | "Vicini S."xsd:string |
| http://purl.uniprot.org/citations/20669324 | http://purl.uniprot.org/core/author | "Swanwick C.C."xsd:string |
| http://purl.uniprot.org/citations/20669324 | http://purl.uniprot.org/core/author | "Shapiro M.E."xsd:string |
| http://purl.uniprot.org/citations/20669324 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20669324 | http://purl.uniprot.org/core/name | "Dev Neurobiol"xsd:string |
| http://purl.uniprot.org/citations/20669324 | http://purl.uniprot.org/core/pages | "875-883"xsd:string |
| http://purl.uniprot.org/citations/20669324 | http://purl.uniprot.org/core/title | "Flotillin-1 promotes formation of glutamatergic synapses in hippocampal neurons."xsd:string |
| http://purl.uniprot.org/citations/20669324 | http://purl.uniprot.org/core/volume | "70"xsd:string |
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