| http://purl.uniprot.org/citations/20670214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20670214 | http://www.w3.org/2000/01/rdf-schema#comment | "SFKs (Src family kinases) are central regulators of many signalling pathways. Their functions are tightly regulated through SH (Src homology) domain-mediated protein-protein interactions. A yeast two-hybrid screen using SH3 domains as bait identified Alix [ALG-2 (apoptosis-linked gene 2)-interacting protein X] as a novel Hck (haemopoietic cell kinase) SH3 domain interactor. The Alix-Hck-SH3 interaction was confirmed in vitro by a GST (glutathione transferase) pull-down assay and in intact cells by a mammalian two-hybrid assay. Furthermore, the interaction was demonstrated to be biologically relevant in cells. Through biophysical experiments, we then identified the PRR (proline-rich region) motif of Alix that binds Hck-SH3 and determined a dissociation constant of 34.5 μM. Heteronuclear NMR spectroscopy experiments were used to map the Hck-SH3 residues that interact with an ALIX construct containing the V and PRR domains or with the minimum identified interacting motif. Finally, SAXS (small-angle X-ray scattering) analysis showed that the N-terminal PRR of Alix is unfolded, at least before Hck-SH3 recognition. These results indicate that residues outside the canonical PxxP motif of Alix enhance its affinity and selectivity towards Hck-SH3. The structural framework of the Hck-Alix interaction will help to clarify how Hck and Alix assist during virus budding and cell-surface receptor regulation."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20100314"xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Morelli X."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Perez J."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Shi X."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Huang M."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Zimmermann P."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Arold S.T."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Collette Y."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Opi S."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Parrot I."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Lugari A."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Corbeil J."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Restouin A."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Madore E."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/author | "Coursindel T."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/name | "Biochem J"xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/pages | "93-102"xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/title | "Identification and biophysical assessment of the molecular recognition mechanisms between the human haemopoietic cell kinase Src homology domain 3 and ALG-2-interacting protein X."xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://purl.uniprot.org/core/volume | "431"xsd:string |
| http://purl.uniprot.org/citations/20670214 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20670214 |
| http://purl.uniprot.org/citations/20670214 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20670214 |
| http://purl.uniprot.org/uniprot/#_A8K4G3-mappedCitation-20670214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20670214 |