| http://purl.uniprot.org/citations/20682852 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20682852 | http://www.w3.org/2000/01/rdf-schema#comment | "Tumor relapse after human leukocyte antigen-matched allogeneic stem cell transplantation (SCT) remains a serious problem, despite the long-term presence of minor histocompatibility antigen (MiHA)-specific memory T cells. Dendritic cell (DC)-based vaccination boosting MiHA-specific T-cell immunity is an appealing strategy to prevent or counteract tumor recurrence, but improvement is necessary to increase the clinical benefit. Here, we investigated whether knockdown of programmed death ligand 1 (PD-L1) and PD-L2 on monocyte-derived DCs results in improved T-cell activation. Electroporation of single siRNA sequences into immature DCs resulted in efficient, specific, and long-lasting knockdown of PD-L1 and PD-L2 expression. PD-L knockdown DCs strongly augmented interferon-γ and interleukin-2 production by stimulated T cells in an allogeneic mixed lymphocyte reaction, whereas no effect was observed on T-cell proliferation. Moreover, we demonstrated that PD-L gene silencing, especially combined PD-L1 and PD-L2 knockdown, resulted in improved proliferation and cytokine production of keyhole limpet hemocyanin-specific CD4(+) T cells. Most importantly, PD-L knockdown DCs showed superior potential to expand MiHA-specific CD8(+) effector and memory T cells from leukemia patients early after donor lymphocyte infusion and later during relapse. These data demonstrate that PD-L siRNA electroporated DCs are highly effective in enhancing T-cell proliferation and cytokine production, and are therefore attractive cells for improving the efficacy of DC vaccines in cancer patients."xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2010-04-278739"xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://purl.uniprot.org/core/author | "de Witte T."xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://purl.uniprot.org/core/author | "Dolstra H."xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://purl.uniprot.org/core/author | "Maas F."xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://purl.uniprot.org/core/author | "van der Voort R."xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://purl.uniprot.org/core/author | "Adisty N."xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://purl.uniprot.org/core/author | "Hobo W."xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://purl.uniprot.org/core/author | "Schaap N."xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20682852 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://purl.uniprot.org/core/pages | "4501-4511"xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://purl.uniprot.org/core/title | "siRNA silencing of PD-L1 and PD-L2 on dendritic cells augments expansion and function of minor histocompatibility antigen-specific CD8+ T cells."xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://purl.uniprot.org/core/volume | "116"xsd:string |
| http://purl.uniprot.org/citations/20682852 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20682852 |
| http://purl.uniprot.org/citations/20682852 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20682852 |
| http://purl.uniprot.org/uniprot/#_B8ZZZ4-mappedCitation-20682852 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20682852 |
| http://purl.uniprot.org/uniprot/#_B3KUU3-mappedCitation-20682852 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20682852 |
| http://purl.uniprot.org/uniprot/#_A4GW21-mappedCitation-20682852 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20682852 |
| http://purl.uniprot.org/uniprot/#_P01732-mappedCitation-20682852 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20682852 |
| http://purl.uniprot.org/uniprot/#_Q2LC89-mappedCitation-20682852 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20682852 |
| http://purl.uniprot.org/uniprot/#_Q0GN75-mappedCitation-20682852 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20682852 |
| http://purl.uniprot.org/uniprot/#_Q6ZVS2-mappedCitation-20682852 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20682852 |
| http://purl.uniprot.org/uniprot/#_Q96QR6-mappedCitation-20682852 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20682852 |