| http://purl.uniprot.org/citations/20688868 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20688868 | http://www.w3.org/2000/01/rdf-schema#comment | "To study positioning of the polypeptide release factor eRF1 toward a stop signal in the ribosomal decoding site, we applied photoactivatable mRNA analogs, derivatives of oligoribonucleotides. The human eRF1 peptides cross-linked to these short mRNAs were identified. Cross-linkers on the guanines at the second, third, and fourth stop signal positions modified fragment 31-33, and to lesser extent amino acids within region 121-131 (the "YxCxxxF loop") in the N domain. Hence, both regions are involved in the recognition of the purines. A cross-linker at the first uridine of the stop codon modifies Val66 near the NIKS loop (positions 61-64), and this region is important for recognition of the first uridine of stop codons. Since the N domain distinct regions of eRF1 are involved in a stop-codon decoding, the eRF1 decoding site is discontinuous and is not of "protein anticodon" type. By molecular modeling, the eRF1 molecule can be fitted to the A site proximal to the P-site-bound tRNA and to a stop codon in mRNA via a large conformational change to one of its three domains. In the simulated eRF1 conformation, the YxCxxxF motif and positions 31-33 are very close to a stop codon, which becomes also proximal to several parts of the C domain. Thus, in the A-site-bound state, the eRF1 conformation significantly differs from those in crystals and solution. The model suggested for eRF1 conformation in the ribosomal A site and cross-linking data are compatible."xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.org/dc/terms/identifier | "doi:10.1261/rna.2066910"xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/author | "Kolosov P.M."xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/author | "Kisselev L.L."xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/author | "Frolova L.Y."xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/author | "Karpova G.G."xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/author | "Ven'yaminova A.G."xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/author | "Graifer D.M."xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/author | "Bulygin K.N."xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/author | "Khairulina Y.S."xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/author | "Vorobjev Y.N."xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/name | "RNA"xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/pages | "1902-1914"xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/title | "Three distinct peptides from the N domain of translation termination factor eRF1 surround stop codon in the ribosome."xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/20688868 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20688868 |
| http://purl.uniprot.org/citations/20688868 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20688868 |
| http://purl.uniprot.org/uniprot/#_B7Z7P8-mappedCitation-20688868 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20688868 |
| http://purl.uniprot.org/uniprot/#_P62495-mappedCitation-20688868 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20688868 |
| http://purl.uniprot.org/uniprot/P62495 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20688868 |
| http://purl.uniprot.org/uniprot/B7Z7P8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20688868 |