| http://purl.uniprot.org/citations/20709853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20709853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20709853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20709853 | http://www.w3.org/2000/01/rdf-schema#comment | "Several microorganisms have been isolated that can transform hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), a cyclic nitramine explosive. To better characterize the microbial genes that facilitate this transformation, we sequenced and annotated a 182-kb plasmid, pGKT2, from the RDX-degrading strain Gordonia sp. KTR9. This plasmid carries xplA, encoding a protein sharing up to 99% amino acid sequence identity with characterized RDX-degrading cytochromes P450. Other genes that cluster with xplA are predicted to encode a glutamine synthase-XplB fusion protein, a second cytochrome P450, Cyp151C, and XplR, a GntR-type regulator. Rhodococcus jostii RHA1 expressing xplA from KTR9 degraded RDX but did not utilize RDX as a nitrogen source. Moreover, an Escherichia coli strain producing XplA degraded RDX but a strain producing Cyp151C did not. KTR9 strains cured of pGKT2 did not transform RDX. Physiological studies examining the effects of exogenous nitrogen sources on RDX degradation in strain KTR9 revealed that ammonium, nitrite, and nitrate each inhibited RDX degradation by up to 79%. Quantitative real-time PCR analysis of glnA-xplB, xplA, and xplR showed that transcript levels were 3.7-fold higher during growth on RDX than during growth on ammonium and that this upregulation was repressed in the presence of various inorganic nitrogen sources. Overall, the results indicate that RDX degradation by KTR9 is integrated with central nitrogen metabolism and that the uptake of RDX by bacterial cells does not require a dedicated transporter."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.org/dc/terms/identifier | "doi:10.1128/AEM.01217-10"xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.01217-10"xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.01217-10"xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Eltis L.D."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Eltis L.D."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Eltis L.D."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Chen H.P."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Chen H.P."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Chen H.P."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Jung C.M."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Jung C.M."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Jung C.M."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Hancock D."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Hancock D."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Hancock D."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Crocker F.H."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Crocker F.H."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Crocker F.H."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Indest K.J."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Indest K.J."xsd:string |
| http://purl.uniprot.org/citations/20709853 | http://purl.uniprot.org/core/author | "Indest K.J."xsd:string |