| http://purl.uniprot.org/citations/20717697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20717697 | http://www.w3.org/2000/01/rdf-schema#comment | "Proliferation and cellular aggregation are both crucial features for survival and self-renewal of primordial germ cells (PGCs). Adhesive proteins play pivotal roles in cell-cell adhesion and signal exchanges under the influence of cytokines, growth factors and bioactive metabolites such as retinoic acid (RA). In this study, proliferation-promoting effect of RA on chicken PGCs was investigated by revealing changes in adhesive proteins E-cadherin and α/β catenins. PGCs were isolated from the genital ridge of 4-day-old chicken embryos and cultured on embryonic fibroblast feeder. RA (10(-7)-10(-5) M) increased PGCs aggregation and mRNA expression of E-cadherin and α/β-catenins. Furthermore, E-cadherin and β-catenin protein expression levels were increased by RA treatment. However, RA-elicited effect was significantly attenuated by a PKC inhibitor H(7). In addition, the number and area of PGC colonies were increased by RA treatment at 10(-7)-10(-5) M. Again, this increase was reduced by combined treatment of H(7). The proliferating effect of RA on PGCs was further confirmed by increased mRNA expression of cyclins, CCND1 and CCNE1, and cyclin-dependent kinases 6 and 2, which are critical for G1-S progression in cell cycle. Moreover, flow cytometry analysis confirmed that RA-treated PGC populations displayed a significant increase in the proportion of S and G2 phase cells. Likewise, this stimulating action was hindered by combined H(7) treatment. These results indicate that RA, as a bioactive metabolite of vitamin A, may promote PGC proliferation and increase intercellular aggregation of PGCs via E-cadherin and α/β-catenins expression through the PKC signaling pathway."xsd:string |
| http://purl.uniprot.org/citations/20717697 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00726-010-0717-x"xsd:string |
| http://purl.uniprot.org/citations/20717697 | http://purl.uniprot.org/core/author | "Zhang C."xsd:string |
| http://purl.uniprot.org/citations/20717697 | http://purl.uniprot.org/core/author | "Yu M."xsd:string |
| http://purl.uniprot.org/citations/20717697 | http://purl.uniprot.org/core/author | "Guan K."xsd:string |
| http://purl.uniprot.org/citations/20717697 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/20717697 | http://purl.uniprot.org/core/name | "Amino Acids"xsd:string |
| http://purl.uniprot.org/citations/20717697 | http://purl.uniprot.org/core/pages | "933-941"xsd:string |
| http://purl.uniprot.org/citations/20717697 | http://purl.uniprot.org/core/title | "The promoting effect of retinoic acid on proliferation of chicken primordial germ cells by increased expression of cadherin and catenins."xsd:string |
| http://purl.uniprot.org/citations/20717697 | http://purl.uniprot.org/core/volume | "40"xsd:string |
| http://purl.uniprot.org/citations/20717697 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20717697 |
| http://purl.uniprot.org/citations/20717697 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20717697 |
| http://purl.uniprot.org/uniprot/#_A0A1D5PQN0-mappedCitation-20717697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20717697 |
| http://purl.uniprot.org/uniprot/#_A0A8V0Z4L2-mappedCitation-20717697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20717697 |
| http://purl.uniprot.org/uniprot/#_O42486-mappedCitation-20717697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20717697 |
| http://purl.uniprot.org/uniprot/#_P08641-mappedCitation-20717697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20717697 |
| http://purl.uniprot.org/uniprot/#_P30997-mappedCitation-20717697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20717697 |
| http://purl.uniprot.org/uniprot/P30997 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20717697 |
| http://purl.uniprot.org/uniprot/A0A1D5PQN0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20717697 |
| http://purl.uniprot.org/uniprot/O42486 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20717697 |
| http://purl.uniprot.org/uniprot/P08641 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20717697 |
| http://purl.uniprot.org/uniprot/A0A8V0Z4L2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20717697 |