| http://purl.uniprot.org/citations/20719962 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20719962 | http://www.w3.org/2000/01/rdf-schema#comment | "Sprouty (Spry) proteins are negative regulators of receptor tyrosine kinase signaling; however, their exact mechanism of action remains incompletely understood. We identified phosphatidylinositol-specific phospholipase C (PLC)-γ as a partner of the Spry1 and Spry2 proteins. Spry-PLCγ interaction was dependent on the Src homology 2 domain of PLCγ and a conserved N-terminal tyrosine residue in Spry1 and Spry2. Overexpression of Spry1 and Spry2 was associated with decreased PLCγ phosphorylation and decreased PLCγ activity as measured by production of inositol (1,4,5)-triphosphate (IP(3)) and diacylglycerol, whereas cells deficient for Spry1 or Spry1, -2, and -4 showed increased production of IP(3) at baseline and further increased in response to growth factor signals. Overexpression of Spry 1 or Spry2 or small-interfering RNA-mediated knockdown of PLCγ1 or PLCγ2 abrogated the activity of a calcium-dependent reporter gene, suggesting that Spry inhibited calcium-mediated signaling downstream of PLCγ. Furthermore, Spry overexpression in T-cells, which are highly dependent on PLCγ activity and calcium signaling, blocked T-cell receptor-mediated calcium release. Accordingly, cultured T-cells from Spry1 gene knockout mice showed increased proliferation in response to T-cell receptor stimulation. These data highlight an important action of Spry, which may allow these proteins to influence signaling through multiple receptors."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e10-02-0123"xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Aggarwal P."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Collins S."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Powell J."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Kim M.K."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Phillips M."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Licht J.D."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Quatela S."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Reddi A.L."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Ambardekar C."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Mason J."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Basson M.A."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Vilimas T."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Akbulut S."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Lovatt M."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Canciani B."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/author | "Hix L."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/pages | "3487-3496"xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/title | "Sprouty proteins inhibit receptor-mediated activation of phosphatidylinositol-specific phospholipase C."xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/20719962 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20719962 |