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http://purl.uniprot.org/citations/20737317http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20737317http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To study the effects of NS398, a selective cyclooxygenase-2 (COX-2) inhibitor, on the transcription and translation of BCL-3 and its regulatory gene cyclin D1 in colon cancer cell line SW480.

Methods

Human colon cancer cells SW480 were divided into two groups: SW480 cells in experimental group were treated with NS398 in different concentrations(25 micromol/L, 50 micromol/L, 100 micromol/L and 200 micromol/L) for 48 h or 72 h. SW480 cells in control group were treated with media which did not contain NS398. Then the expressions of BCL-3 and cyclin D1 were detected by RT-PCR, Western blot, and immunocytochemistry.

Results

At 48 hours RT-PCR showed that BCL-3 mRNA and cyclin D1 mRNA decreased in a dose-dependent manner in the experimental group. However, there were no significant differences in the levels of BCL-3 protein and cyclin D1 protein between two groups (P>0.05). At 72 hours, BCL-3 protein and cyclin D1 protein also decreased in a dose-dependent manner in the experimental group. When the concentration of NS398 reached 100 micromol/L, the differences between the two groups in the expression of BCL-3 mRNA and protein became statistically significant (P<0.01). When the concentration of NS398 reached 50 micromol/L, the differences in the expression of cyclin D1 mRNA and protein were statistically significant (P<0.05).

Conclusions

BCL-3 is expressed in colon cancer cell line SW480. COX-2 inhibitor can inhibit the expression of BCL-3 and cyclin D1 in a dose-dependent manner. NS398 may down-regulate the expression of cyclin D1 through BCL-3."xsd:string
http://purl.uniprot.org/citations/20737317http://purl.uniprot.org/core/author"Yang G."xsd:string
http://purl.uniprot.org/citations/20737317http://purl.uniprot.org/core/author"Wu X."xsd:string
http://purl.uniprot.org/citations/20737317http://purl.uniprot.org/core/author"Ye M."xsd:string
http://purl.uniprot.org/citations/20737317http://purl.uniprot.org/core/author"Wang S.M."xsd:string
http://purl.uniprot.org/citations/20737317http://purl.uniprot.org/core/author"Ni S.M."xsd:string
http://purl.uniprot.org/citations/20737317http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20737317http://purl.uniprot.org/core/name"Zhonghua Wei Chang Wai Ke Za Zhi"xsd:string
http://purl.uniprot.org/citations/20737317http://purl.uniprot.org/core/pages"612-615"xsd:string
http://purl.uniprot.org/citations/20737317http://purl.uniprot.org/core/title"[Effect of COX-2 inhibitor on the expression of BCL-3 and cyclin D1 in human colon cancer cell line SW480]."xsd:string
http://purl.uniprot.org/citations/20737317http://purl.uniprot.org/core/volume"13"xsd:string
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