| http://purl.uniprot.org/citations/20800622 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20800622 | http://www.w3.org/2000/01/rdf-schema#comment | "Etoposide is a widely used anticancer drug in the treatment of different tumors. Etoposide is known to activate a wide range of intracellular signals, which may in turn induce cellular responses other than apoptosis. ADAM10 and TACE/ADAM17 belong to a family of transmembrane extracellular metalloproteinases involved in paracrine/juxtacrine regulation of many signaling pathways. The aim of this work was to evaluate if etoposide induces upregulation of ADAM10 or TACE/ADAM17 in two cell lines (GC-1 and GC-2) derived from male germ cells. Results showed that etoposide induced apoptosis in a dose-response manner in both GC-1 and GC-2 cells. Apoptosis started to increase 6h after etoposide addition in GC-2 cells, whereas the same was observed 18h after addition to the GC-1 cells. Protein and mRNA levels of ADAM10 and TACE/ADAM17 increased 18h after etoposide was removed from the GC-1 cells. In GC-2 cells, the protein levels of both proteins increased 12h after etoposide was removed. ADAM10 mRNA increased after 3h and then steadily decreased up to 12h after removal, whereas TACE/ADAM17 mRNA decreased after etoposide removal. Finally, apoptosis was prevented in GC-1 and GC-2 cells by the addition of pharmacological inhibitors of ADAM10 and TACE/ADAM17 to the culture medium of etoposide-treated cells. Our results show for the first time that etoposide upregulates ADAM10 and TACE/ADAM17 mRNA and protein levels. In addition, we also show that ADAM10 and TACE/ADAM17 have a role in etoposide-induced apoptosis."xsd:string |
| http://purl.uniprot.org/citations/20800622 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbamcr.2010.08.003"xsd:string |
| http://purl.uniprot.org/citations/20800622 | http://purl.uniprot.org/core/author | "Ludwig A."xsd:string |
| http://purl.uniprot.org/citations/20800622 | http://purl.uniprot.org/core/author | "Moreno R.D."xsd:string |
| http://purl.uniprot.org/citations/20800622 | http://purl.uniprot.org/core/author | "Lizama C."xsd:string |
| http://purl.uniprot.org/citations/20800622 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/20800622 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/20800622 | http://purl.uniprot.org/core/pages | "120-128"xsd:string |
| http://purl.uniprot.org/citations/20800622 | http://purl.uniprot.org/core/title | "Etoposide induces apoptosis and upregulation of TACE/ADAM17 and ADAM10 in an in vitro male germ cell line model."xsd:string |
| http://purl.uniprot.org/citations/20800622 | http://purl.uniprot.org/core/volume | "1813"xsd:string |
| http://purl.uniprot.org/citations/20800622 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20800622 |
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