| http://purl.uniprot.org/citations/20805474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20805474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20805474 | http://www.w3.org/2000/01/rdf-schema#comment | "Chronic myeloid leukemia (CML) is genetically characterized by the Philadelphia (Ph) chromosome, formed through a reciprocal translocation between chromosomes 9 and 22 and giving rise to the constitutively active tyrosine kinase P210 BCR/ABL1. Therapeutic strategies aiming for a cure of CML will require full eradication of Ph chromosome-positive (Ph(+)) CML stem cells. Here we used gene-expression profiling to identify IL-1 receptor accessory protein (IL1RAP) as up-regulated in CML CD34(+) cells and also in cord blood CD34(+) cells as a consequence of retroviral BCR/ABL1 expression. To test whether IL1RAP expression distinguishes normal (Ph(-)) and leukemic (Ph(+)) cells within the CML CD34(+)CD38(-) cell compartment, we established a unique protocol for conducting FISH on small numbers of sorted cells. By using this method, we sorted cells directly into drops on slides to investigate their Ph-chromosome status. Interestingly, we found that the CML CD34(+)CD38(-)IL1RAP(+) cells were Ph(+), whereas CML CD34(+)CD38(-)IL1RAP(-) cells were almost exclusively Ph(-). By performing long-term culture-initiating cell assays on the two cell populations, we found that Ph(+) and Ph(-) candidate CML stem cells could be prospectively separated. In addition, by generating an anti-IL1RAP antibody, we provide proof of concept that IL1RAP can be used as a target on CML CD34(+)CD38(-) cells to induce antibody-dependent cell-mediated cytotoxicity. This study thus identifies IL1RAP as a unique cell surface biomarker distinguishing Ph(+) from Ph(-) candidate CML stem cells and opens up a previously unexplored avenue for therapy of CML."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1004408107"xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1004408107"xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Richter J."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Richter J."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Olofsson T."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Olofsson T."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Hansen N."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Hansen N."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Rissler M."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Rissler M."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Fioretos T."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Fioretos T."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Lassen C."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Lassen C."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Agerstam H."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Agerstam H."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Bjerrum O.W."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Bjerrum O.W."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Jaeraas M."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Jaeraas M."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Johnels P."xsd:string |
| http://purl.uniprot.org/citations/20805474 | http://purl.uniprot.org/core/author | "Johnels P."xsd:string |