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http://purl.uniprot.org/citations/20811643http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20811643http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20811643http://www.w3.org/2000/01/rdf-schema#comment"

Background

Rnd3 (RhoE) protein belongs to the unique branch of Rho family GTPases that has low intrinsic GTPase activity and consequently remains constitutively active [1], [2]. The current consensus is that Rnd1 and Rnd3 function as important antagonists of RhoA signaling primarily by activating the ubiquitous p190 RhoGAP [3], but not by inhibiting the ROCK family kinases.

Methodology/principal findings

Rnd3 is abundant in mouse embryonic stem (mES) cells and in an unbiased two-step affinity purification screen we identified a new Rnd3 target, termed synectin-binding RhoA exchange factor (Syx), by mass spectrometry. The Syx interaction with Rnd3 does not occur through the Syx DH domain but utilizes a region similar to the classic Raf1 Ras-binding domain (RBD), and most closely related to those in RGS12 and RGS14. We show that Syx behaves as a genuine effector of Rnd3 (and perhaps Rnd1), with binding characteristics similar to p190-RhoGAP. Morpholino-oligonucleotide knockdown of Syx in zebrafish at the one cell stage resulted in embryos with shortened anterior-posterior body axis: this phenotype was effectively rescued by introducing mouse Syx1b mRNA. A Rnd3-binding defective mutant of Syx1b mutated in the RBD (E164A/R165D) was more potent in rescuing the embryonic defects than wild-type Syx1b, showing that Rnd3 negatively regulates Syx activity in vivo.

Conclusions/significance

This study uncovers a well defined Rnd3 effector Syx which is widely expressed and directly impacts RhoA activation. Experiments conducted in vivo indicate that Rnd3 negatively regulates Syx, and that as a RhoA-GEF it plays a key role in early embryonic cell shape changes. Thus a connection to signaling via the planar cell polarity pathway is suggested."xsd:string
http://purl.uniprot.org/citations/20811643http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0012409"xsd:string
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/author"Manser E."xsd:string
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/author"Manser E."xsd:string
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/author"Goh L.L."xsd:string
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/author"Goh L.L."xsd:string
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/name"PLoS ONE"xsd:string
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/name"PLoS One"xsd:string
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/pages"e12409"xsd:string
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/pages"e12409"xsd:string
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/title"The RhoA GEF Syx is a target of Rnd3 and regulated via a Raf1-like ubiquitin-related domain."xsd:string
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/title"The RhoA GEF Syx is a target of Rnd3 and regulated via a Raf1-like ubiquitin-related domain."xsd:string
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/volume"5"xsd:string
http://purl.uniprot.org/citations/20811643http://purl.uniprot.org/core/volume"5"xsd:string
http://purl.uniprot.org/citations/20811643http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20811643
http://purl.uniprot.org/citations/20811643http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20811643
http://purl.uniprot.org/citations/20811643http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/20811643
http://purl.uniprot.org/citations/20811643http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/20811643
http://purl.uniprot.org/uniprot/Q6PC71http://purl.uniprot.org/core/citationhttp://purl.uniprot.org/citations/20811643
http://purl.uniprot.org/uniprot/Q6DHG5http://purl.uniprot.org/core/citationhttp://purl.uniprot.org/citations/20811643
http://purl.uniprot.org/uniprot/A0A8M9Q3T8#attribution-2F3652F76FEB5E1EAC02346C5E7F4000http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/20811643
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