| http://purl.uniprot.org/citations/20817008 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20817008 | http://www.w3.org/2000/01/rdf-schema#comment | "Hepatic stellate cells (HSCs) have a critical role in liver physiology, and in the pathogenesis of liver inflammation and fibrosis. Here, we investigated the interplay between leukotrienes (LT) and TGF-β in the activation mechanisms of HSCs from schistosomal granulomas (GR-HSCs). First, we demonstrated that GR-HSCs express 5-lipoxygenase (5-LO), as detected by immunolocalization in whole cells and confirmed in cell lysates through western blotting and by mRNA expression through RT-PCR. Moreover, mRNA expression of 5-LO activating protein (FLAP) and LTC(4)-synthase was also documented, indicating that GR-HSCs have the molecular machinery required for LT synthesis. Morphological analysis of osmium and Oil-Red O-stained HSC revealed large numbers of small lipid droplets (also known as lipid bodies). We observed co-localization of lipid droplet protein marker (ADRP) and 5-LO by immunofluorescence microscopy. We demonstrated that GR-HSCs were able to spontaneously release cysteinyl-LTs (CysLTs), but not LTB(4,) into culture supernatants. CysLT production was highly enhanced after TGF-β-stimulation. Moreover, the 5-LO inhibitor zileuton and 5-LO gene deletion were able to inhibit the TGF-β-stimulated proliferation of GR-HSCs, suggesting a role for LTs in HSC activation. Here, we extend the immunoregulatory function of HSC by demonstrating that HSC from liver granulomas of schistosome-infected mouse are able to release Cys-LTs in a TGF-β-regulated manner, potentially impacting pathogenesis and liver fibrosis in schistosomiasis."xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbalip.2010.08.014"xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/author | "Bozza P.T."xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/author | "Perez S.A."xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/author | "Bandeira-Melo C."xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/author | "Borojevic R."xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/author | "Silva P.M."xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/author | "El-Cheikh M.C."xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/author | "Maya-Monteiro C.M."xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/author | "Paiva L.A."xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/author | "Teodoro A.J."xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/pages | "1341-1348"xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/title | "Interplay of cysteinyl leukotrienes and TGF-beta in the activation of hepatic stellate cells from Schistosoma mansoni granulomas."xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://purl.uniprot.org/core/volume | "1801"xsd:string |
| http://purl.uniprot.org/citations/20817008 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20817008 |
| http://purl.uniprot.org/citations/20817008 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20817008 |
| http://purl.uniprot.org/uniprot/#_E9QA93-mappedCitation-20817008 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20817008 |
| http://purl.uniprot.org/uniprot/#_A0A0N4SW45-mappedCitation-20817008 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20817008 |
| http://purl.uniprot.org/uniprot/#_E9Q6H6-mappedCitation-20817008 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20817008 |
| http://purl.uniprot.org/uniprot/#_P48999-mappedCitation-20817008 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20817008 |
| http://purl.uniprot.org/uniprot/#_Q3TC68-mappedCitation-20817008 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20817008 |
| http://purl.uniprot.org/uniprot/#_Q3TBK8-mappedCitation-20817008 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20817008 |