| http://purl.uniprot.org/citations/20818336 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20818336 | http://www.w3.org/2000/01/rdf-schema#comment | "Myocardin-related transcription factors (MRTFs) are actin-regulated transcriptional coactivators, which bind G-actin through their N-terminal RPEL domains. In response to signal-induced actin polymerisation and concomitant G-actin depletion, MRTFs accumulate in the nucleus and activate target gene transcription through their partner protein SRF. Nuclear accumulation of MRTFs in response to signal is inhibited by increased G-actin level. Here, we study the mechanism by which MRTF-A enters the nucleus. We show that MRTF-A contains an unusually long bipartite nuclear localisation signal (NLS), comprising two basic elements separated by 30 residues, embedded within the RPEL domain. Using siRNA-mediated protein depletion in vivo, and nuclear import assays in vitro, we show that the MRTF-A extended bipartite NLS uses the importin (Imp)α/β-dependent import pathway, and that import is inhibited by G-actin. Interaction of the NLS with the Impα-Impβ heterodimer requires both NLS basic elements, and is dependent on the Impα major and minor binding pockets. Binding of the Impα-Impβ heterodimer to the intact MRTF-A RPEL domain occurs competitively with G-actin. Thus, MRTF-A contains an actin-sensitive nuclear import signal."xsd:string |
| http://purl.uniprot.org/citations/20818336 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2010.216"xsd:string |
| http://purl.uniprot.org/citations/20818336 | http://purl.uniprot.org/core/author | "Treisman R."xsd:string |
| http://purl.uniprot.org/citations/20818336 | http://purl.uniprot.org/core/author | "Vartiainen M.K."xsd:string |
| http://purl.uniprot.org/citations/20818336 | http://purl.uniprot.org/core/author | "Rajakyla E.K."xsd:string |
| http://purl.uniprot.org/citations/20818336 | http://purl.uniprot.org/core/author | "Pawlowski R."xsd:string |
| http://purl.uniprot.org/citations/20818336 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20818336 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/20818336 | http://purl.uniprot.org/core/pages | "3448-3458"xsd:string |
| http://purl.uniprot.org/citations/20818336 | http://purl.uniprot.org/core/title | "An actin-regulated importin alpha/beta-dependent extended bipartite NLS directs nuclear import of MRTF-A."xsd:string |
| http://purl.uniprot.org/citations/20818336 | http://purl.uniprot.org/core/volume | "29"xsd:string |
| http://purl.uniprot.org/citations/20818336 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20818336 |
| http://purl.uniprot.org/citations/20818336 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20818336 |
| http://purl.uniprot.org/uniprot/O35345#attribution-B3AC4CDAC1B475CE3460354EBF4BCFAF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20818336 |
| http://purl.uniprot.org/uniprot/Q14974#attribution-F1187F63CBC42D7280A69E627EAE6106 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20818336 |
| http://purl.uniprot.org/uniprot/O35343#attribution-B3AC4CDAC1B475CE3460354EBF4BCFAF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20818336 |
| http://purl.uniprot.org/uniprot/C0LLJ0#attribution-B3AC4CDAC1B475CE3460354EBF4BCFAF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20818336 |
| http://purl.uniprot.org/uniprot/O35344#attribution-B3AC4CDAC1B475CE3460354EBF4BCFAF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20818336 |
| http://purl.uniprot.org/uniprot/O00629#attribution-D0DF9EE517AD2F74090BAD6E783F1AB4 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20818336 |
| http://purl.uniprot.org/uniprot/O00629#attribution-F1187F63CBC42D7280A69E627EAE6106 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20818336 |
| http://purl.uniprot.org/uniprot/#_A0A0B4J1E7-mappedCitation-20818336 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20818336 |
| http://purl.uniprot.org/uniprot/#_D3Z0I2-mappedCitation-20818336 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20818336 |
| http://purl.uniprot.org/uniprot/#_A0A5F8MQ33-mappedCitation-20818336 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20818336 |
| http://purl.uniprot.org/uniprot/#_A0A668KL76-mappedCitation-20818336 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20818336 |