| http://purl.uniprot.org/citations/20826819 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20826819 | http://www.w3.org/2000/01/rdf-schema#comment | "In Saccharomyces cerevisiae, some of the nascent chains can be post-translationally translocated into the endoplasmic reticulum through the heptameric post-translational translocon complex (post-translocon). This membrane-protein complex is composed of the protein-conducting channel and the tetrameric Sec62/63 complex. The Sec62/63 complex plays crucial roles in targeting of the signal recognition particle-independent protein substrate to the protein-conducting channel and in assembly of the post-translocon. Although the molecular mechanism of the post-translational translocation process has been well established, the structure of the post-translocon and how the channel and the Sec62/63 complex form the heptameric complex are largely uncharacterized. Here, we report a 20-Å resolution cryo-electron microscopy structure of the post-translocon. The purified post-translocon was found to have a mass of 287 kDa, which is consistent with the unit stoichiometry of the seven subunits as determined by a cysteine labeling experiment. We demonstrated that Triton X-100 dissociated the heptameric complex into three subcomplexes identified as the trimeric translocon Sec61-Sbh1-Sss1, the Sec63-Sec71-Sec72 trimer, and the heterotetramer Sec62-Sec63-Sec71-Sec72, respectively. Additionally, a role of the sixth cytosolic loop of Sec61 in assembly of the post-translocon was demonstrated. Mutations of conserved, positively charged amino acid residues in the loop caused decreased formation of the post-translocon. These studies provide the first architectural description of the yeast post-translocon."xsd:string |
| http://purl.uniprot.org/citations/20826819 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m110.159517"xsd:string |
| http://purl.uniprot.org/citations/20826819 | http://purl.uniprot.org/core/author | "Li H."xsd:string |
| http://purl.uniprot.org/citations/20826819 | http://purl.uniprot.org/core/author | "Li H.'"xsd:string |
| http://purl.uniprot.org/citations/20826819 | http://purl.uniprot.org/core/author | "Wall J.S."xsd:string |
| http://purl.uniprot.org/citations/20826819 | http://purl.uniprot.org/core/author | "Harada Y."xsd:string |
| http://purl.uniprot.org/citations/20826819 | http://purl.uniprot.org/core/author | "Lennarz W.J."xsd:string |
| http://purl.uniprot.org/citations/20826819 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/20826819 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/20826819 | http://purl.uniprot.org/core/pages | "2956-2965"xsd:string |
| http://purl.uniprot.org/citations/20826819 | http://purl.uniprot.org/core/title | "Structural studies and the assembly of the heptameric post-translational translocon complex."xsd:string |
| http://purl.uniprot.org/citations/20826819 | http://purl.uniprot.org/core/volume | "286"xsd:string |
| http://purl.uniprot.org/citations/20826819 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20826819 |
| http://purl.uniprot.org/citations/20826819 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20826819 |
| http://purl.uniprot.org/uniprot/P35179#attribution-06524EE44A8C81760562845624128EE6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20826819 |
| http://purl.uniprot.org/uniprot/P52870#attribution-06524EE44A8C81760562845624128EE6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20826819 |
| http://purl.uniprot.org/uniprot/P32915#attribution-06524EE44A8C81760562845624128EE6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20826819 |
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| http://purl.uniprot.org/uniprot/P21825#attribution-CC8AD938AAB2EF5047971B60AF9E45BF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20826819 |
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| http://purl.uniprot.org/uniprot/P33754#attribution-CC8AD938AAB2EF5047971B60AF9E45BF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20826819 |
| http://purl.uniprot.org/uniprot/P14906#attribution-06524EE44A8C81760562845624128EE6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20826819 |
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| http://purl.uniprot.org/uniprot/P39742#attribution-06524EE44A8C81760562845624128EE6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20826819 |