| http://purl.uniprot.org/citations/20833133 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20833133 | http://www.w3.org/2000/01/rdf-schema#comment | "Neutrophils contribute significantly to ALI (acute lung injury) through adhesion to pulmonary microvascular endothelial cells (PMEC), trans-PMEC migration and alveolar infiltration. Trans-PMEC migration delays expression of neutrophil apoptosis, which promotes intra-alveolar neutrophil survival and neutrophil mediated ALI. We assessed the role of neutrophil vs PMEC inducible nitric oxide (NO) synthase (iNOS) in modulating neutrophil apoptosis. Apoptosis of wild-type vs iNOS-/-neutrophils was quantified by microscopy and FACS annexin-V binding. In a murine model of ALI, neutrophils isolated by BAL(broncho-alveolar lavage) from iNOS-/-mice had increased expression of apoptosis after 24h culture ex vivo than wild-type neutrophils (15.2±3.3 vs 3.0±0.4%, mean±sd, p<0.01). Apoptosis rates of isolated bone marrow iNOS+/+ vs iNOS-/-neutrophils were similar under basal and LPS/IFN-γ stimulation, and following LPS/IFN-γ-stimulated trans-PMEC migration. Apoptosis of both iNOS+/+ and iNOS-/-neutrophils was inhibited by trans-PMEC migration only across iNOS+/+ PMEC (1.6±0.3 and 1.5±0.3%, respectively; p<0.05 for each vs non-migrated neutrophils) but not across iNOS-/-PMEC (4.3±1 and 3.1±0.6%, respectively). PMEC iNOS-dependent inhibition of neutrophil apoptosis was independent of changes in neutrophil caspase-3 activity. We conclude that PMEC iNOS, but not neutrophil iNOS, has an important inhibitory effect on neutrophil apoptosis during trans-PMEC neutrophil migration, which is independent of caspase-3 activity. Further studies will define the mechanism of PMEC iNOS-dependent inhibition of neutrophil apoptosis and assess the potential relevance of this phenomenon in human neutrophils and ALI."xsd:string |
| http://purl.uniprot.org/citations/20833133 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2010.09.029"xsd:string |
| http://purl.uniprot.org/citations/20833133 | http://purl.uniprot.org/core/author | "Mehta S."xsd:string |
| http://purl.uniprot.org/citations/20833133 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
| http://purl.uniprot.org/citations/20833133 | http://purl.uniprot.org/core/author | "Taneja R."xsd:string |
| http://purl.uniprot.org/citations/20833133 | http://purl.uniprot.org/core/author | "Law C."xsd:string |
| http://purl.uniprot.org/citations/20833133 | http://purl.uniprot.org/core/author | "Gillis C."xsd:string |
| http://purl.uniprot.org/citations/20833133 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20833133 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/20833133 | http://purl.uniprot.org/core/pages | "207-212"xsd:string |
| http://purl.uniprot.org/citations/20833133 | http://purl.uniprot.org/core/title | "Modulation of neutrophil apoptosis by murine pulmonary microvascular endothelial cell inducible nitric oxide synthase."xsd:string |
| http://purl.uniprot.org/citations/20833133 | http://purl.uniprot.org/core/volume | "401"xsd:string |
| http://purl.uniprot.org/citations/20833133 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20833133 |
| http://purl.uniprot.org/citations/20833133 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20833133 |
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| http://purl.uniprot.org/uniprot/#_P29477-mappedCitation-20833133 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833133 |
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| http://purl.uniprot.org/uniprot/D3YC69 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20833133 |