| http://purl.uniprot.org/citations/20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20833718 | http://www.w3.org/2000/01/rdf-schema#comment | "Very small amounts of MHC class II-peptide complexes expressed on the surface of antigen-presenting cells (APCs) are capable of stimulating antigen-specific CD4 T cells. There is intense interest to elucidate the molecular mechanisms by which these small amounts of MHC-II can cluster, cross-link T cell receptors, and promote T cell proliferation. We now demonstrate that a significant fraction of the total pool of MHC-II molecules on the surface of dendritic cells is physically associated in macromolecular aggregates. These MHC-II/MHC-II interactions have been probed by co-immunoprecipitation analysis of the MHC-II I-A molecule with the related I-E molecule. These molecular associations are maintained in gentle detergents but are disrupted in harsh detergents such as Triton X-100. MHC-II I-A/I-E interactions are disrupted when plasma membrane cholesterol is extracted using methyl β-cyclodextrin, suggesting that lipid raft microdomains are important mediators of these MHC-II interactions. Although it has been proposed that tetraspanin proteins regulate molecular clustering, aggregation, and co-immunoprecipitation in APCs, genetic deletion of the tetraspanin family members CD9 or CD81 had no effect on MHC-II I-A/I-E binding. These data demonstrate that the presence of distinct forms of MHC-II with plasma membrane lipid rafts is required for MHC-II aggregation in APCs and provides a molecular mechanism allowing dendritic cells expressing small amounts of MHC-II-peptide complexes to cross-link and stimulate CD4 T cells."xsd:string |
| http://purl.uniprot.org/citations/20833718 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m110.147793"xsd:string |
| http://purl.uniprot.org/citations/20833718 | http://purl.uniprot.org/core/author | "Roche P.A."xsd:string |
| http://purl.uniprot.org/citations/20833718 | http://purl.uniprot.org/core/author | "Khandelwal S."xsd:string |
| http://purl.uniprot.org/citations/20833718 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20833718 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/20833718 | http://purl.uniprot.org/core/pages | "35303-35310"xsd:string |
| http://purl.uniprot.org/citations/20833718 | http://purl.uniprot.org/core/title | "Distinct MHC class II molecules are associated on the dendritic cell surface in cholesterol-dependent membrane microdomains."xsd:string |
| http://purl.uniprot.org/citations/20833718 | http://purl.uniprot.org/core/volume | "285"xsd:string |
| http://purl.uniprot.org/citations/20833718 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20833718 |
| http://purl.uniprot.org/citations/20833718 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A1D8M9B3-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A0H3TRK9-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A2Z4T131-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A2Z4T140-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A2Z4T188-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A2Z4T196-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A2Z4T1A9-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A2Z4T1Z3-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A2Z4T225-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A2Z4T259-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A2Z4T282-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A2Z4T2C0-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |
| http://purl.uniprot.org/uniprot/#_A0A2Z4T337-mappedCitation-20833718 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20833718 |