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| http://purl.uniprot.org/citations/20844140 | http://www.w3.org/2000/01/rdf-schema#comment | "At the mammalian skeletal neuromuscular junction, cycling of nicotinic ACh receptors (nAChRs) is critical for the maintenance of a high postsynaptic receptor density. However, the mechanisms that regulate nAChRs recycling in living animals remain unknown. Using in vivo time-lapse imaging, fluorescence recovery after photobleaching, and biochemical pull down assays, we demonstrated that recycling of internalized nAChRs into fully functional and denervated synapses was promoted by both direct muscle stimulation and pharmacologically induced intracellular calcium elevations. Most of internalized nAChRs are recycled directly into synaptic sites. Chelating of intracellular calcium below resting level drastically decreased cycling of nAChRs. Furthermore we found that calcium-dependent AChR recycling is mediated by Ca(2+)/calmodulin-dependent kinase II (CaMKII). Inhibition of CaMKII selectively blocked recycling and caused intracellular accumulation of internalized nAChRs, whereas internalization of surface receptors remained unaffected. Electroporation of CaMKII-GFP isoforms into the sternomastoid muscle showed that muscle-specific CaMKIIβm isoform is highly expressed at the neuromuscular junction (NMJ) and precisely colocalized with nAChRs at crests of synaptic folds while the CaMKIIγ and δ isoforms are poorly expressed in synaptic sites. These results indicate that Ca(2+) along with CaMKII activity are critical for receptor recycling and may provide a mechanism by which the postsynaptic AChR density is maintained at the NMJ in vivo."xsd:string |
| http://purl.uniprot.org/citations/20844140 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.3309-10.2010"xsd:string |
| http://purl.uniprot.org/citations/20844140 | http://purl.uniprot.org/core/author | "Mouslim C."xsd:string |
| http://purl.uniprot.org/citations/20844140 | http://purl.uniprot.org/core/author | "Akaaboune M."xsd:string |
| http://purl.uniprot.org/citations/20844140 | http://purl.uniprot.org/core/author | "Martinez-Pena y Valenzuela I."xsd:string |
| http://purl.uniprot.org/citations/20844140 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20844140 | http://purl.uniprot.org/core/name | "J Neurosci"xsd:string |
| http://purl.uniprot.org/citations/20844140 | http://purl.uniprot.org/core/pages | "12455-12465"xsd:string |
| http://purl.uniprot.org/citations/20844140 | http://purl.uniprot.org/core/title | "Calcium/calmodulin kinase II-dependent acetylcholine receptor cycling at the mammalian neuromuscular junction in vivo."xsd:string |
| http://purl.uniprot.org/citations/20844140 | http://purl.uniprot.org/core/volume | "30"xsd:string |
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