| http://purl.uniprot.org/citations/20845358 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20845358 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20845358 | http://www.w3.org/2000/01/rdf-schema#comment | "Human liver-expressed antimicrobial peptide 2 (LEAP-2) is a cationic antimicrobial peptide (CAMP) believed to have a protective role against bacterial infection. Little is known about the structure-activity relationships of LEAP-2 or its mechanism of action. In this study we describe the structure of LEAP-2, analyze its interaction with model membranes, and relate them to the antimicrobial activity of the peptide. The structure of LEAP-2, determined by NMR spectroscopy, reveals a compact central core with disorder at the N and C termini. The core comprises a β-hairpin and a 3(10)-helix that are braced by disulfide bonds between Cys17-28 and Cys23-33 and further stabilized by a network of hydrogen bonds. Membrane-affinity studies show that LEAP-2 membrane binding is governed by electrostatic attractions, which are sensitive to ionic strength. Truncation studies show that the C-terminal region of LEAP-2 is irrelevant for membrane binding, whereas the N-terminal (hydrophobic domain) and core regions (cationic domain) are essential. Bacterial-growth-inhibition assays reveal that the antimicrobial activity of LEAP-2 correlates with membrane affinity. Interestingly, the native and reduced forms of LEAP-2 have similar membrane affinity and antimicrobial activities; this suggests that disulfide bonds are not essential for the bactericidal activity. This study reveals that LEAP-2 has a novel fold for a CAMP and suggests that although LEAP-2 exhibits antimicrobial activity under low-salt conditions, there is likely to be another physiological role for the peptide."xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.org/dc/terms/identifier | "doi:10.1002/cbic.201000400"xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.org/dc/terms/identifier | "doi:10.1002/cbic.201000400"xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/author | "Craik D.J."xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/author | "Craik D.J."xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/author | "Clark R.J."xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/author | "Clark R.J."xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/author | "Henriques S.T."xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/author | "Henriques S.T."xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/author | "Tan C.C."xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/author | "Tan C.C."xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/name | "ChemBioChem"xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/name | "ChemBioChem"xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/pages | "2148-2157"xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/pages | "2148-2157"xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/title | "Structural and functional analysis of human liver-expressed antimicrobial peptide 2."xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/title | "Structural and functional analysis of human liver-expressed antimicrobial peptide 2."xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/20845358 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20845358 |
| http://purl.uniprot.org/citations/20845358 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20845358 |