| http://purl.uniprot.org/citations/20857263 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20857263 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20857263 | http://www.w3.org/2000/01/rdf-schema#comment | "We recently reported the chromosome region maintenance 1 (CRM1)-dependent nuclear export of intron-less human interferon-α1 (IFN-α1) mRNA, which encodes a main effecter of host innate immunity. We show that the coding region of IFN-α1 mRNA forms novel secondary structures that are responsible for the CRM1-dependent export of the transcript. Deletion-mutagenesis, in vivo export assays, and computer analyses of the folding potentials of export-competent fragments revealed the presence of a domain, termed the conserved secondary structure (CSS), comprising two adjacent putative stable stem-loop structures (nt 208-452). Internal deletion-mutagenesis and constitutive export assays of each stem-loop structure demonstrated that subregions 308-322 and 352-434 act as a core element by conferring the export function on the CSS. Leptomycin B (LMB) inhibition of the CRM1 pathway decreased the export of core element RNA, implying that the principal site of CRM1 action for exporting IFN-α1 mRNA resides within the core element. An RNPS1 (RNA-binding protein S1, serine-rich domain) cDNA was isolated by yeast three-hybrid screening, using bait containing two CSS regions. We showed that RNPS1 might recognize IFN-α1 mRNP that includes CRM1. The data demonstrate that interaction between RNA structures in the coding region and CRM1 affects the nucleocytoplasmic translocation of IFN-α1 mRNA."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00795-010-0492-5"xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00795-010-0492-5"xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/author | "Ito S."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/author | "Ito S."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/author | "Kimura T."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/author | "Kimura T."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/author | "Nishizawa M."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/author | "Nishizawa M."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/author | "Yamada H."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/author | "Yamada H."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/author | "Hashimoto I."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/author | "Hashimoto I."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/name | "Med. Mol. Morphol."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/name | "Med. Mol. Morphol."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/pages | "145-157"xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/pages | "145-157"xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/title | "Novel cis-active structures in the coding region mediate CRM1-dependent nuclear export of IFN-alpha 1 mRNA."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/title | "Novel cis-active structures in the coding region mediate CRM1-dependent nuclear export of IFN-alpha 1 mRNA."xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/volume | "43"xsd:string |
| http://purl.uniprot.org/citations/20857263 | http://purl.uniprot.org/core/volume | "43"xsd:string |