| http://purl.uniprot.org/citations/20861010 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20861010 | http://www.w3.org/2000/01/rdf-schema#comment | "The activity of phosphodiesterase-5 (PDE5) is specific for cGMP and is regulated by cGMP binding to GAF-A in its regulatory domain. To better understand the regulatory mechanism, x-ray crystallographic and biochemical studies were performed on constructs of human PDE5A1 containing the N-terminal phosphorylation segment, GAF-A, and GAF-B. Superposition of this unliganded GAF-A with the previously reported NMR structure of cGMP-bound PDE5 revealed dramatic conformational differences and suggested that helix H4 and strand B3 probably serve as two lids to gate the cGMP-binding pocket in GAF-A. The structure also identified an interfacial region among GAF-A, GAF-B, and the N-terminal loop, which may serve as a relay of the cGMP signal from GAF-A to GAF-B. N-terminal loop 98-147 was physically associated with GAF-B domains of the dimer. Biochemical analyses showed an inhibitory effect of this loop on cGMP binding and its involvement in the cGMP-induced conformation changes."xsd:string |
| http://purl.uniprot.org/citations/20861010 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m110.141614"xsd:string |
| http://purl.uniprot.org/citations/20861010 | http://purl.uniprot.org/core/author | "Ke H."xsd:string |
| http://purl.uniprot.org/citations/20861010 | http://purl.uniprot.org/core/author | "Robinson H."xsd:string |
| http://purl.uniprot.org/citations/20861010 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/20861010 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20861010 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/20861010 | http://purl.uniprot.org/core/pages | "38149-38156"xsd:string |
| http://purl.uniprot.org/citations/20861010 | http://purl.uniprot.org/core/title | "Conformation changes, N-terminal involvement, and cGMP signal relay in the phosphodiesterase-5 GAF domain."xsd:string |
| http://purl.uniprot.org/citations/20861010 | http://purl.uniprot.org/core/volume | "285"xsd:string |
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