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http://purl.uniprot.org/citations/20869761http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20869761http://www.w3.org/2000/01/rdf-schema#comment"

Objective

This study examined the biological and clinical significance of NAC1 expression in ovarian cancer and assessed whether NAC1 has the potential to be a therapeutic target.

Methods

NAC1 expression and gene amplification were assessed in ovarian cancers by immunohistochemistry, fluorescence in situ hybridization, and clinical data collected by a retrospective chart review. NAC1 gene knockdown using silencing RNA and a NAC1 gene transfection system were used to assess NAC1 function in ovarian cancer tissue samples.

Results

The frequency of positive NAC1 expression in serous adenocarcinomas (50.0%:22/44) was significantly higher than that in the other histological subtypes (33.3%: 10/30). NAC1 gene amplification was identified in seven (9.5%) of 74 ovarian carcinomas. Positive NAC1 expression significantly correlated with shorter disease-free and overall survival (P = 0.002, P = 0.0048). A multivariate analysis showed that positive NAC1 expression was an independent prognostic factor for disease-free and overall survival after standard platinum-taxane chemotherapy (P = 0.0027, P = 0.0302). Profound growth inhibition, increased apoptosis, decreased cell proliferation, and decreased cell migration and invasion were observed in silencing RNA-treated cancer cells with NAC1 overexpression compared with cancer cells without NAC1 expression. NAC1 overexpression stimulated proliferation, migration, and invasion in ovarian cancer cell lines KF28 and TOV-21G, which normally lacked NAC1 expression.

Conclusion

These findings indicate that NAC1 over-expression is critical to the growth and survival of ovarian cancers. Furthermore, they suggest that NAC1 silencing RNA-induced phenotypes depend on the expression status of the targeted cell line. Therefore, NAC1-targeted therapy may benefit ovarian cancer patients with NAC1 expression."xsd:string
http://purl.uniprot.org/citations/20869761http://purl.org/dc/terms/identifier"doi:10.1016/j.ygyno.2010.08.031"xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/author"Iida K."xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/author"Nakayama K."xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/author"Rahman M.T."xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/author"Miyazaki K."xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/author"Nakayama N."xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/author"Ishikawa M."xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/author"Rahman M."xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/author"Yeasmin S."xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/author"Katagiri A."xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/name"Gynecol Oncol"xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/pages"469-478"xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/title"Biological role and prognostic significance of NAC1 in ovarian cancer."xsd:string
http://purl.uniprot.org/citations/20869761http://purl.uniprot.org/core/volume"119"xsd:string
http://purl.uniprot.org/citations/20869761http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20869761
http://purl.uniprot.org/citations/20869761http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/20869761
http://purl.uniprot.org/uniprot/#_Q96RE7-mappedCitation-20869761http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/20869761
http://purl.uniprot.org/uniprot/Q96RE7http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/20869761